ArticleMolecular and cellular biology1995
Synaptic and epidermal accumulations of human acetylcholinesterase are encoded by alternative 3'-terminal exons.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 80 citations in OpenAlex.
- Article
- Characterisation of cholinesterases in mucous secretions and their localisation in epidermis of Labeo rohita and Cirrhinus mrigala.Fish physiology and biochemistry · 2019Article
- Alzheimer's disease: Targeting the Cholinergic System.Current neuropharmacology · 2016Review
- New treatments for myasthenia: a focus on antisense oligonucleotides.Drug design, development and therapy · 2013Review
- Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility.Journal of cellular and molecular medicine · 2008Article
- Plant-derived human acetylcholinesterase-R provides protection from lethal organophosphate poisoning and its chronic aftermath.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2007Article
- The RNA-binding protein HuD binds acetylcholinesterase mRNA in neurons and regulates its expression after axotomy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- ARP, the cleavable C-terminal peptide of "readthrough" acetylcholinesterase, promotes neuronal development and plasticity.Journal of molecular neuroscience : MN · 2006Review
- Article
- Changes in neuronal acetylcholinesterase gene expression and division of labor in honey bee colonies.Journal of molecular neuroscience : MN · 2001Article
- Excess "read-through" acetylcholinesterase attenuates but the "synaptic" variant intensifies neurodeterioration correlates.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Stability and secretion of acetylcholinesterase forms in skeletal muscle cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Human osteogenesis involves differentiation-dependent increases in the morphogenically active 3' alternative splicing variant of acetylcholinesterase.Molecular and cellular biology · 1999Article
- Functional redundancy of acetylcholinesterase and neuroligin in mammalian neuritogenesis.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Acetylcholinesterase enhances neurite growth and synapse development through alternative contributions of its hydrolytic capacity, core protein, and variable C termini.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Acetylcholinesterase-transgenic mice display embryonic modulations in spinal cord choline acetyltransferase and neurexin Ibeta gene expression followed by late-onset neuromotor deterioration.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Normal and atypical butyrylcholinesterases in placental development, function, and malfunction.Cellular and molecular neurobiology · 1997Article
- Synaptogenesis and myopathy under acetylcholinesterase overexpression.Journal of molecular neuroscience : MNArticle
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue-specific heterogeneity among mammalian acetylcholinesterases (AChE) has been associated with 3' alternative splicing of the primary AChE gene transcript. We have previously demonstrated that human AChE DNA encoding the brain and muscle AChE form and bearing the 3' exon E6 (ACHE-E6) induces accumulation of catalytically active AChE in myotomes and neuromuscular junctions (NMJs) of 2- and 3-day-old Xenopus embryos. Here, we explore the possibility that the 3'-terminal exons of two alternative human AChE cDNA constructs include evolutionarily conserved tissue-recognizable elements. To this end, DNAs encoding alternative human AChE mRNAs were microinjected into cleaving embryos of Xenopus laevis. In contrast to the myotomal expression demonstrated by ACHE-E6, DNA carrying intron 14 and alternative exon E5 (ACHE-I4/E5) promoted punctuated staining of epidermal cells and secretion of AChE into the external medium. Moreover, ACHE-E6-injected embryos displayed enhanced NMJ development, whereas ACHE-I4/E5-derived enzyme was conspicuously absent from muscles and NMJs and its expression in embryos had no apparent effect on NMJ development. In addition, cell-associated AChE from embryos injected with ACHE-I4/E5 DNA was biochemically distinct from that encoded by the muscle-expressible ACHE-E6, displaying higher electrophoretic mobility and greater solubility in low-salt buffer. These findings suggest that alternative 3'-terminal exons dictate tissue-specific accumulation and a particular biological role(s) of AChE, associate the 3' exon E6 with NMJ development, and indicate the existence of a putative secretory AChE form derived from the alternative I4/E5 AChE mRNA.
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