ArticleMolecular and cellular biology1995
EFIA/YB-1 is a component of cardiac HF-1A binding activity and positively regulates transcription of the myosin light-chain 2v gene.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 69 citations in OpenAlex.
- Characterization of cis-regulatory elements and transcription factor binding: gel mobility shift assay.Methods in molecular biology (Clifton, N.J.) · 2007Article
- YB-1 promotes strand separation in vitro of duplex DNA containing either mispaired bases or cisplatin modifications, exhibits endonucleolytic activities and binds several DNA repair proteins.Nucleic acids research · 2004Article
- Calreticulin reveals a critical Ca(2+) checkpoint in cardiac myofibrillogenesis.The Journal of cell biology · 2002Article
- Carp, a cardiac ankyrin-repeated protein, and its new homologue, Arpp, are differentially expressed in heart, skeletal muscle, and rhabdomyosarcomas.The American journal of pathology · 2002Article
- Ablation of Cypher, a PDZ-LIM domain Z-line protein, causes a severe form of congenital myopathy.The Journal of cell biology · 2001Article
- The role of interleukin-1 in the failing heart.Heart failure reviews · 2001Review
- Modulation of MLC-2v gene expression by AP-1: complex regulatory role of Jun in cardiac myocytes.Molecular and cellular biochemistry · 2001Article
- Physical and functional interaction between the Y-box binding protein YB-1 and human polyomavirus JC virus large T antigen.Journal of virology · 1999Article
- Reciprocal interaction between two cellular proteins, Puralpha and YB-1, modulates transcriptional activity of JCVCY in glial cells.Molecular and cellular biology · 1999Article
- A positive GATA element and a negative vitamin D receptor-like element control atrial chamber-specific expression of a slow myosin heavy-chain gene during cardiac morphogenesis.Molecular and cellular biology · 1998Article
- The cardiac tissue-restricted homeobox protein Csx/Nkx2.5 physically associates with the zinc finger protein GATA4 and cooperatively activates atrial natriuretic factor gene expression.Molecular and cellular biology · 1998Article
- Myocyte-specific enhancer factor 2 and thyroid hormone receptor associate and synergistically activate the alpha-cardiac myosin heavy-chain gene.Molecular and cellular biology · 1997Article
- Suppression of grp78 core promoter element-mediated stress induction by the dbpA and dbpB (YB-1) cold shock domain proteins.Molecular and cellular biology · 1997Article
- Combinatorial control of muscle development by basic helix-loop-helix and MADS-box transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 1996Review
- Transcriptional control of a nuclear gene encoding a mitochondrial fatty acid oxidation enzyme in transgenic mice: role for nuclear receptors in cardiac and brown adipose expression.Molecular and cellular biology · 1996Article
- Review
- Inhibition of a signaling pathway in cardiac muscle cells by active mitogen-activated protein kinase kinase.Molecular biology of the cell · 1995Article
- Catecholamines and cardiac growth.Molecular and cellular biochemistryArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Transient assays in cultured ventricular muscle cells and studies in transgenic mice have identified two adjacent regulatory elements (HF-1a and HF-1b/MEF-2) as required to maintain ventricular chamber-specific expression of the myosin light-chain 2v (MLC-2v) gene. A rat neonatal heart cDNA library was screened with an HF-1a binding site, resulting in the isolation of EFIA, the rat homolog of human YB-1. Purified recombinant EFIA/YB-1 protein binds to the HF-1a site in a sequence-specific manner and contacts a subset of the HF-1a contact points made by the cardiac nuclear factor(s). The HF-1a sequence contains AGTGG, which is highly homologous to the inverted CCAAT core of the EFIA/YB-1 binding sites and is found to be essential for binding of the recombinant EFIA/YB-1. Antiserum against Xenopus YB-3 (100% identical in the DNA binding domain and 89% identical in overall amino acid sequence to rat EFIA) can specifically abolish a component of the endogenous HF-1a complex in the rat cardiac myocyte nuclear extracts. In cotransfection assays, EFIA/YB-1 increased 250-bp MLC-2v promoter activity by 3.4-fold specifically in the cardiac cell context and in an HF-1a site-dependent manner. EFIA/YB-1 complexes with an unknown protein in cardiac myocyte nuclear extracts to form the endogenous HF-1a binding activity. Immunocoprecipitation revealed that EFIA/YB-1 has a major associated protein of approximately 30 kDa (p30) in cardiac muscle cells. This study suggests that EFIA/YB-1, together with the partner p30, binds to the HF-1a site and, in conjunction with HF-1b/MEF-2, mediates ventricular chamber-specific expression of the MLC-2v gene.
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