ArticleDiabetologia1995
Intracellular localization and molecular heterogeneity of the sulphonylurea receptor in insulin-secreting cells.
Article in Diabetologia, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 71 citations in OpenAlex.
- Interaction of sulphonylurea derivatives with vascular ATP-sensitive potassium channels in humans.Diabetologia · 1996Trial
- Organelle-Resolved Tetrazine-trans-Cyclooctene Click Chemistry for Cargo Delivery and Release.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- New Aspects of Diabetes Research and Therapeutic Development.Pharmacological reviews · 2021Review
- Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men.Physiological reviews · 2018Review
- A Novel Diphenylthiosemicarbazide Is a Potential Insulin Secretagogue for Anti-Diabetic Agen.PloS one · 2016Article
- K(ATP) channels and islet hormone secretion: new insights and controversies.Nature reviews. Endocrinology · 2013Review
- Cell signalling in insulin secretion: the molecular targets of ATP, cAMP and sulfonylurea.Diabetologia · 2012Review
- α-Synuclein binds the K(ATP) channel at insulin-secretory granules and inhibits insulin secretion.American journal of physiology. Endocrinology and metabolism · 2011Article
- Human K(ATP) channelopathies: diseases of metabolic homeostasis.Pflugers Archiv : European journal of physiology · 2010Review
- Morphological localisation of sulfonylurea receptor 1 in endocrine cells of human, mouse and rat pancreas.Diabetologia · 2007Article
- Intracellular ATP-sensitive K+ channels in mouse pancreatic beta cells: against a role in organelle cation homeostasis.Diabetologia · 2006Article
- Sulphonylurea action revisited: the post-cloning era.Diabetologia · 2003Review
- Nuclear KATP channels trigger nuclear Ca(2+) transients that modulate nuclear function.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The stimulatory action of tolbutamide on Ca2+-dependent exocytosis in pancreatic beta cells is mediated by a 65-kDa mdr-like P-glycoprotein.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Aspects of novel sites of regulation of the insulin stimulus-secretion coupling in normal and diabetic pancreatic islets.Endocrine · 1998Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Sulphonylureas stimulate insulin secretion by binding to a receptor in the pancreatic beta-cell plasma membrane resulting in inhibition of ATP-sensitive K+ channels, membrane depolarization and thus influx of Ca2+ through voltage-dependent Ca2+ channels. Sulphonylureas can also induce hormone release at fixed membrane potentials without Ca2+ entry suggesting that these drugs may have other modes of action. We have determined whether different forms of sulphonylurea-binding proteins are present in insulin-secreting cells and their subcellular localization by density gradient centrifugation. Binding studies using [3H]-glibenclamide showed that islet and insulinoma membranes contained a single high affinity sulphonylurea binding site (Kd = 1 nmol/l). Photo-crosslinking of the drug to the membranes resulted in labelling of two proteins with apparent molecular weights of 170 and 140 kDa. The same analyses of insulinoma subcellular fractions showed that the majority (> 90%) of binding proteins were localized to intracellular membranes with only minor levels (< 10%) on plasma membranes. The 170 kDa sulphonylurea binding protein was present in both plasma and granule membrane fractions whereas the 140 kDa form was not present in the plasma membrane fraction. The differences in the molecular forms and subcellular distribution of the receptor are consistent with sulphonylureas having multiple sites of action in the pancreatic beta cell.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.