ArticleIn vitro cellular & developmental biology. Animal1995
Growth factor regulation of proliferation in primary cultures of small intestinal epithelium.
Article in In vitro cellular & developmental biology. Animal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Article
- Isolation, culture, and identification of duck intestinal epithelial cells and oxidative stress model constructed.In vitro cellular & developmental biology. Animal · 2019Article
- Efficient Generation of Small Intestinal Epithelial-like Cells from Human iPSCs for Drug Absorption and Metabolism Studies.Stem cell reports · 2018Article
- Amniotic fluid: Source of trophic factors for the developing intestine.World journal of gastrointestinal pathophysiology · 2016Review
- Characteristic and functional analysis of a newly established porcine small intestinal epithelial cell line.PloS one · 2014Article
- Intestinal epithelial cells in vitro.Stem cells and development · 2010Review
- Host factors in amniotic fluid and breast milk that contribute to gut maturation.Clinical reviews in allergy & immunology · 2008Review
- Establishment of fetal bovine intestinal epithelial cell cultures susceptible to bovine rotavirus infection.Journal of virological methods · 2008Article
- Primary culture of colonocytes in rotating bioreactor.In vitro cellular & developmental biology. Animal · 1998Article
- Cytokines in inflammatory bowel disease.Mediators of inflammation · 1997Article
- Establishment of a long-term culture system for rat colon epithelial cells.In vitro cellular & developmental biology. AnimalArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the intestinal epithelium is one of the most rapidly renewing tissues, little is known about the major growth factors that control the rate of cell replacement and migration. Recently, a primary culture model has been described for the developing rat small intestinal epithelium, which permits epithelial growth while maintaining interactions with associated stromal cells, thereby possessing several contextual advantages over established cell lines (Evans et al., 1992). We have used this model to begin to determine the factors that may be involved in controlling intestinal epithelial cell proliferation. Under the conditions examined, no single growth factor promoted exclusive proliferation of epithelial cells; stromal cell proliferation was also apparent. The most potent stimulators of epithelial proliferation were insulin and insulin-like growth factor 1 (IGF-1). These factors also appeared to inhibit migration of the epithelial cells. 5-10 ng/ml EGF, 5-20 ng/ml TGF alpha, and 10-20 ng/ml PDGF also slightly increased epithelial cell numbers. Cell proliferation was inhibited by 0.1 ng/ml TGF beta-1. In Dulbecco's modified Eagle's medium (DMEM) containing 0.25 IU/ml insulin, glucose levels of 2-3 g/liter permitted epithelial growth with limited expansion of the stromal cell population. Higher levels of glucose further stimulated the nonepithelial cell types. Transferrin was also a potent stimulator of both cell types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.