ArticleIn vitro cellular & developmental biology. Animal1995
Effects of VIP on the regulation of mucin secretion in cultured human pancreatic cancer cells (Capan-1).
Article in In vitro cellular & developmental biology. Animal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 16 citations in OpenAlex.
- Mucin dynamics and enteric pathogens.Nature reviews. Microbiology · 2011Review
- Spatio-temporal localization of vasoactive intestinal peptide and neutral endopeptidase in allergic murine lungs.Regulatory peptides · 2010Article
- The absence of the VPAC(2) receptor does not protect mice from Aspergillus induced allergic asthma.Peptides · 2010Article
- Mucins in the mucosal barrier to infection.Mucosal immunology · 2008Review
- Mucin dynamics in intestinal bacterial infection.PloS one · 2008Article
- Cerebellar cortical-layer-specific control of neuronal migration by pituitary adenylate cyclase-activating polypeptide.Neuroscience · 2007Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of Vasoactive intestinal peptide (VIP) on mucin secretion in the pancreatic cancer Capan-1 cell line were studied by Enzyme-linked-immunosorbent-assay (ELISA), and by light and electron microscopy using immunocytological methods. During the exponential growth phase, mucins were accumulated in the cytoplasm of cells and slowly exocytosed. In contrast, there was enhanced exocytosis of mucins during the stationary phase when the cells were well-polarized. Moreover, during this phase, VIP induced a dose-dependent rise in mucin content in the extracellular medium. The reaction with anti-M1 monoclonal antibodies, which recognize specifically the peptide core of gastric mucins, showed an accumulation of secretion granules near the apex of well-polarized cells together with fusion of the granule and plasma membranes after VIP stimulation. Moreover, mucin exocytosis was stimulated by Pituitary adenylate cyclase activating polypeptide (PACAP) and secretin. It was also increased after forskolin treatment suggesting that this mechanism was cAMP-dependent. Our results suggested that exocytosis of mucins could be under the control of VIP in pancreatic duct cells of the Capan-1 cell line.
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