ArticleBiophysical journal1995
Mechanical properties of neuronal growth cone membranes studied by tether formation with laser optical tweezers.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 144 papers.
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Who cites it
144 citing papers in PubMed, 396 citations in OpenAlex.
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- Optical tweezers in biomedical research - progress and techniques.Journal of medicine and life · 2024Review
- Nanomagnetic Guidance Shapes the Structure-Function Relationship of Developing Cortical Networks.Nano letters · 2024Article
- Plasma membrane abundance dictates phagocytic capacity and functional cross-talk in myeloid cells.Science immunology · 2024Article
- Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function.Nature immunology · 2024Article
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- Protrusion growth driven by myosin-generated force.Developmental cell · 2023Article
- How sticky? How tight? How hot? Imaging probes for fluid viscosity, membrane tension and temperature measurements at the cellular level.The international journal of biochemistry & cell biology · 2022Review
- Membrane tension propagation couples axon growth and collateral branching.Science advances · 2022Article
- The Role of Cytoskeleton Revealed by Quartz Crystal Microbalance and Digital Holographic Microscopy.International journal of molecular sciences · 2022Article
- Deep learning assisted mechanotyping of individual cells through repeated deformations and relaxations in undulating channels.Biomicrofluidics · 2022Article
- Alpha-mangostin dephosphorylates ERM to induce adhesion and decrease surface stiffness in KG-1 cells.Human cell · 2022Article
- Mechanosurveillance: Tiptoeing T Cells.Frontiers in immunology · 2022Review
- Mechanical stiffness softening and cell adhesion are coordinately regulated by ERM dephosphorylation in KG-1 cells.Human cell · 2021Article
- Dynamic interplay between cell membrane tension and clathrin-mediated endocytosis.Biology of the cell · 2021Review
- Review
84 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many cell phenomena involve major morphological changes, particularly in mitosis and the process of cell migration. For cells or neuronal growth cones to migrate, they must extend the leading edge of the plasma membrane as a lamellipodium or filopodium. During extension of filopodia, membrane must move across the surface creating shear and flow. Intracellular biochemical processes driving extension must work against the membrane mechanical properties, but the forces required to extend growth cones have not been measured. In this paper, laser optical tweezers and a nanometer-level analysis system were used to measure the neuronal growth cone membrane mechanical properties through the extension of filopodia-like tethers with IgG-coated beads. Although the probability of a bead attaching to the membrane was constant irrespective of treatment; the probability of forming a tether with a constant force increased dramatically with cytochalasin B or D and dimethylsulfoxide (DMSO). These are treatments that alter the organization of the actin cytoskeleton. The force required to hold a tether at zero velocity (F0) was greater than forces generated by single molecular motors, kinesin and myosin; and F0 decreased with cytochalasin B or D and DMSO in correlation with the changes in the probability of tether formation. The force of the tether on the bead increased linearly with the velocity of tether elongation. From the dependency of tether force on velocity of tether formation, we calculated a parameter related to membrane viscosity, which decreased with cytochalasin B or D, ATP depletion, nocodazole, and DMSO. These results indicate that the actin cytoskeleton affects the membrane mechanical properties, including the force required for membrane extension and the viscoelastic behavior.
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