ArticleBiophysical journal1995
Cell-substrate interactions and locomotion of Dictyostelium wild-type and mutants defective in three cytoskeletal proteins: a study using quantitative reflection interference contrast microscopy.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 80 citations in OpenAlex.
- Critical role of lipid membranes in polarization and migration of cells: a biophysical view.Biophysical reviews · 2021Review
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- Understanding dynamic changes in live cell adhesion with neutron reflectometry.Modern physics letters. B, Condensed matter physics, statistical physics, applied physics · 2014Article
- Both contractile axial and lateral traction force dynamics drive amoeboid cell motility.The Journal of cell biology · 2014Article
- Calreticulin affects cell adhesiveness through differential phosphorylation of insulin receptor substrate-1.Cellular & molecular biology letters · 2014Article
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- Impact of the carbazole derivative wiskostatin on mechanical stability and dynamics of motile cells.Journal of muscle research and cell motility · 2012Article
- Chemotactic cell trapping in controlled alternating gradient fields.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The role of cell contraction and adhesion in dictyostelium motility.Biophysical journal · 2010Article
- Mouse fibroblast cell adhesion studied by neutron reflectometry.Biophysical journal · 2010Article
- "Shimmying like my sister" cell.Biophysical journal · 2008Article
- Diffusion and deformations of single hydra cells in cellular aggregates.Biophysical journal · 2000Article
- A micromechanic study of cell polarity and plasma membrane cell body coupling in Dictyostelium.Biophysical journal · 2000Article
- Electrically excitable normal rat kidney fibroblasts: A new model system for cell-semiconductor hybrids.Biophysical journal · 1999Article
- A role for Dictyostelium racE in cortical tension and cleavage furrow progression.The Journal of cell biology · 1998Article
- Membrane bending modulus and adhesion energy of wild-type and mutant cells of Dictyostelium lacking talin or cortexillins.Biophysical journal · 1998Article
- Talin-null cells of Dictyostelium are strongly defective in adhesion to particle and substrate surfaces and slightly impaired in cytokinesis.The Journal of cell biology · 1997Article
- Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes.Molecular biology of the cell · 1997Article
- Dictyostelium discoideum cells lacking the 34,000-dalton actin-binding protein can grow, locomote, and develop, but exhibit defects in regulation of cell structure and movement: a case of partial redundancy.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reflection interference contrast microscopy combined with digital image processing was applied to study the motion of Dictyostelium discoideum cells in their pre-aggregative state on substrata of different adhesiveness (glass, albumin-covered glass, and freshly cleaved mica). The temporal variations of the size and shape of the cell/substratum contact area and the time course of advancement of pseudopods protruding in contact with the substratum were analyzed. The major goal was to study differences between the locomotion of wild-type cells and strains of triple mutants deficient in two F-actin cross-linking proteins (alpha-actinin and the 120-kDa gelation factor) and one F-actin fragmenting protein (severin). The size of contact area, AC, of both wild-type and mutant cells fluctuates between minimum and maximum values on the order of minutes, pointing toward an intrinsic switching mechanism associated with the mechanochemical control system. The fluctuation amplitudes are much larger on freshly cleaved mica than on glass. Wild-type and mutant cells exhibit remarkable differences on mica but not on glass. These differences comprise the population median of AC and alterations in pseudopod protrusion. AC is smaller by a factor of two or more for all mutants. Pseudopods protrude slower and shorter in the mutants. It is concluded that cell shape and pseudopods are destabilized by defects in the actin-skeleton, which can be overcompensated by strongly adhesive substrata. Several features of amoeboid cell locomotion on substrata can be understood on the basis of the minimum bending energy concept of soft adhering shells and by assuming that adhesion induces local alterations of the composite membrane consisting of the protein/lipid bilayer on the cell surface and the underlying actin-cortex.
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