ArticleProceedings of the National Academy of Sciences of the United States of America1995
Two nuclear localization signals present in the basic-helix 1 domains of MyoD promote its active nuclear translocation and can function independently.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 56 citations in OpenAlex.
- Identification and Functional Analysis of Dual Nuclear Localization Signals on Desmin.ACS omega · 2026Article
- 3D photopolymerized microstructured scaffolds influence nuclear deformation, nucleo/cytoskeletal protein organization, and gene regulation in mesenchymal stem cells.APL bioengineering · 2023Article
- Exon skipping in genes encoding lineage-defining myogenic transcription factors in rhabdomyosarcoma.Cold Spring Harbor molecular case studies · 2022Article
- Helical structure motifs made searchable for functional peptide design.Nature communications · 2022Article
- Mechanical regulation of nucleocytoplasmic translocation in mesenchymal stem cells: characterization and methods for investigation.Biophysical reviews · 2019Review
- Recessive MYF5 Mutations Cause External Ophthalmoplegia, Rib, and Vertebral Anomalies.American journal of human genetics · 2018Article
- Nandrolone-induced nuclear accumulation of MyoD protein is mediated by Numb, a Notch inhibitor, in C2C12 myoblasts.Physiological reports · 2018Article
- A novel MCPH1 isoform complements the defective chromosome condensation of human MCPH1-deficient cells.PloS one · 2012Article
- hMSH5 is a nucleocytoplasmic shuttling protein whose stability depends on its subcellular localization.Nucleic acids research · 2010Article
- Multiple nuclear localization signals function in the nuclear import of the transcription factor Nrf2.The Journal of biological chemistry · 2008Article
- Nuclear import of PKCdelta is required for apoptosis: identification of a novel nuclear import sequence.The EMBO journal · 2002Article
- Specific induction of Z-DNA conformation by a nuclear localization signal peptide of lupin glutaminyl tRNA synthetase.Molecular biology reports · 2000Article
- Discrimination between NL1- and NL2-mediated nuclear localization of the glucocorticoid receptor.Molecular and cellular biology · 1999Article
- Cytomegalovirus assembly protein precursor and proteinase precursor contain two nuclear localization signals that mediate their own nuclear translocation and that of the major capsid protein.Journal of virology · 1998Article
- Intramolecular regulation of MyoD activation domain conformation and function.Molecular and cellular biology · 1998Article
- Nuclear import and export of influenza virus nucleoprotein.Journal of virology · 1997Article
- Preferential MyoD homodimer formation demonstrated by a general method of dominant negative mutation employing fusion with a lysosomal protease.The Journal of cell biology · 1996Article
- Accumulation in fetal muscle and localization to the neuromuscular junction of cAMP-dependent protein kinase A regulatory and catalytic subunits RI alpha and C alpha.The Journal of cell biology · 1996Article
- The cytostatic function of c-Abl is controlled by multiple nuclear localization signals and requires the p53 and Rb tumor suppressor gene products.The EMBO journal · 1996Article
- The bovine papillomavirus type 1 E2 transactivator and repressor proteins use different nuclear localization signals.Journal of virology · 1996Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MyoD, a member of the family of helix-loop-helix myogenic factors that plays a crucial role in skeletal muscle differentiation, is a nuclear phosphoprotein. Using microinjection of purified MyoD protein into rat fibroblasts, we show that the nuclear import of MyoD is a rapid and active process, being ATP and temperature dependent. Two nuclear localization signals (NLSs), one present in the basic region and the other in the helix 1 domain of MyoD protein, are demonstrated to be functional in promoting the active nuclear transport of MyoD. Synthetic peptides spanning these two NLSs and biochemically coupled to IgGs can promote the nuclear import of microinjected IgG conjugates in muscle and nonmuscle cells. Deletion analysis reveals that each sequence can function independently within the MyoD protein since concomittant deletion of both sequences is required to alter the nuclear import of this myogenic factor. In addition, the complete cytoplasmic retention of a beta-galactosidase-MyoD fusion mutant protein, double deleted at these two NLSs, argues against the existence of another functional NLS motif in MyoD.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.