ArticleProceedings of the National Academy of Sciences of the United States of America1995
Bombesin and [Leu8]phyllolitorin promote fetal mouse lung branching morphogenesis via a receptor-mediated mechanism.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 81 citations in OpenAlex.
- Mechanostimulation of neuroendocrine cells and their clinical relevance.Molecular and cellular biochemistry · 2026Review
- Bombesins: A New Frontier in Hybrid Compound Development.Pharmaceutics · 2023Review
- Urine gastrin-releasing peptide in the first week correlates with bronchopulmonary dysplasia and post-prematurity respiratory disease.Pediatric pulmonology · 2020Observational
- [Clinical features of neuroendocrine cell hyperplasia of infancy].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2020Article
- Comparative Transcriptome Analysis During the Seven Developmental Stages of Channel Catfish (Frontiers in genetics · 2020Article
- Fgf10/Fgfr2b Signaling Orchestrates the Symphony of Molecular, Cellular, and Physical Processes Required for Harmonious Airway Branching Morphogenesis.Frontiers in cell and developmental biology · 2020Review
- Progesterone receptor isoform B expression in pulmonary neuroendocrine cells decreases cell proliferation.The Journal of steroid biochemistry and molecular biology · 2019Article
- Oxygen, gastrin-releasing Peptide, and pediatric lung disease: life in the balance.Frontiers in pediatrics · 2014Review
- Defective pulmonary innervation and autonomic imbalance in congenital diaphragmatic hernia.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- Gastrin-releasing peptide blockade as a broad-spectrum anti-inflammatory therapy for asthma.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Bombesin-like peptides modulate alveolarization and angiogenesis in bronchopulmonary dysplasia.American journal of respiratory and critical care medicine · 2007Article
- Bombesin inhibits alveolarization and promotes pulmonary fibrosis in newborn mice.American journal of respiratory and critical care medicine · 2006Article
- Immunomodulatory functions of the diffuse neuroendocrine system: implications for bronchopulmonary dysplasia.Endocrine pathology · 2004Review
- Mapping the distribution of neuroepithelial bodies of the rat lung. A whole-mount immunohistochemical approach.The American journal of pathology · 1997Article
- Pulmonary Neuroendocrine Cells and Lung Development.Endocrine pathology · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Pulmonary neuroendocrine cells are localized predominantly at airway branchpoints. Previous work showed that gastrin-releasing peptide (GRP), a major pulmonary bombesin-like peptide, occurred in neuroendocrine cells exclusively in branching human fetal airways. We now demonstrate that GRP and GRP receptor genes are expressed in fetal mouse lung as early as embryonic day 12 (E12), when lung buds are beginning to branch. By in situ hybridization, GRP receptor transcripts were at highest levels in mesenchymal cells at cleft regions of branching airways and blood vessels. To explore the possibility that bombesin-like peptides might play a role in branching morphogenesis, E12 lung buds were cultured for 48 hr in serum-free medium. In the presence of 0.10-10 microM bombesin, branching was significantly augmented as compared with control cultures, with a peak of 94% above control values at 1 microM (P < 0.005). The bombesin receptor antagonist [Leu13- psi(CH2NH)Leu14]bombesin alone (100 nM) had no effect on baseline branching but completely abolished bombesin-induced branching. A bombesin-related peptide, [Leu8]phyllolitorin also increased branching (65% above control values at 10 nM, P < 0.005). [Leu8]Phyllolitorin also significantly augmented thymidine incorporation in cultured lung buds. Fibronectin, which is abundant at branchpoints, induces GRP gene expression in undifferentiated cell lines. These observations suggest that BLPs secreted by pulmonary neuroendocrine cells may contribute to lung branching morphogenesis. Furthermore, components of branchpoints may induce pulmonary neuroendocrine cell differentiation as part of a positive feedback loop, which could account in part for the high prevalence of these cells at branchpoints.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.