ArticleProceedings of the National Academy of Sciences of the United States of America1995
Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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Who cites it
31 citing papers in PubMed, 67 citations in OpenAlex.
- Collective unstructured interactions drive chromatin binding of transcription factors.bioRxiv : the preprint server for biology · 2025Article
- RANBP17 Overexpression Restores Nucleocytoplasmic Transport and Ameliorates Neurodevelopment in Induced DYT1 Dystonia Motor Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Nuclear pore complex and nucleocytoplasmic transport disruption in neurodegeneration.FEBS letters · 2023Review
- RANBP1 (RAN Binding Protein 1): The Missing Genetic Piece in Cancer Pathophysiology and Other Complex Diseases.Cancers · 2023Review
- Nuclear pore complexes - a doorway to neural injury in neurodegeneration.Nature reviews. Neurology · 2022Review
- Types of nuclear localization signals and mechanisms of protein import into the nucleus.Cell communication and signaling : CCS · 2021Review
- Karyopherins regulate nuclear pore complex barrier and transport function.The Journal of cell biology · 2017Article
- Virtual Nuclear Envelope Breakdown and Its Regulators in Fission Yeast Meiosis.Frontiers in cell and developmental biology · 2016Review
- Conserved spatial organization of FG domains in the nuclear pore complex.Biophysical journal · 2013Article
- The importin β binding domain as a master regulator of nucleocytoplasmic transport.Biochimica et biophysica acta · 2011Review
- Regucalcin and cell regulation: role as a suppressor protein in signal transduction.Molecular and cellular biochemistry · 2011Review
- Phosphorylation meets nuclear import: a review.Cell communication and signaling : CCS · 2010Article
- Transportin mediates nuclear entry of DNA in vertebrate systems.Traffic (Copenhagen, Denmark) · 2009Article
- Nuclear import of hepatitis B virus capsids and release of the viral genome.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Localization of importin alpha (Rch1) at the plasma membrane and subcellular redistribution during lymphocyte activation.Chromosoma · 2003Article
- Characterization of DRGs, developmentally regulated GTP-binding proteins, from pea and Arabidopsis.Plant molecular biology · 1999Article
- Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT In vitro.The Journal of cell biology · 1998Article
- The balance of RanBP1 and RCC1 is critical for nuclear assembly and nuclear transport.Molecular biology of the cell · 1997Article
- RanGTP-mediated nuclear export of karyopherin alpha involves its interaction with the nucleoporin Nup153.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Nucleocytoplasmic transport of macromolecules.Microbiology and molecular biology reviews : MMBR · 1997Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclei of digitonin-permeabilized cells that had been preloaded with a model transport substrate in a cytosol-dependent import reaction were subsequently incubated to investigate which conditions would result in export of transport substrate. We found that up to 80% of the imported substrate was exported when recombinant human Ran and GTP were present in the export reaction. Ran-mediated export was inhibited by nonhydrolyzable GTP analogs and also by wheat germ agglutinin but was unaffected by a nonhydrolyzable ATP analog. Moreover, a recombinant human Ran mutant that was deficient in its GTPase activity inhibited export. These data indicate that export of proteins from the nucleus requires Ran and GTP hydrolysis but not ATP hydrolysis. We also found that digitonin-permeabilized cells were depleted of their endogenous nuclear Ran, thus allowing detection of Ran as a limiting factor for export. In contrast, most endogenous karyopherin alpha was retained in nuclei of digitonin-permeabilized cells. Unexpectedly, exogenously added, fluorescently labeled Ran, although it accessed the nuclear interior, was found to dock at the nuclear rim in a punctate pattern, suggesting the existence of Ran-binding sites at the nuclear pore complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.