ArticleNucleic acids research1995
Detection of platinum-DNA adducts by 32P-postlabelling.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 30 citations in OpenAlex.
- Cell-cycle-specific lesion evolution rather than inhibition of double-strand-break repair underpins cisplatin radiosensitization.Molecular oncology · 2026Article
- 3D-DIP-Chip: a microarray-based method to measure genomic DNA damage.Scientific reports · 2015Article
- Personalized medicine for targeted and platinum-based chemotherapy of lung and bladder cancer.Bioanalysis · 2013Review
- A microdosing approach for characterizing formation and repair of carboplatin-DNA monoadducts and chemoresistance.International journal of cancer · 2011Article
- Transplatin-conjugated triplex-forming oligonucleotides form adducts with both strands of DNA.Bioconjugate chemistry · 2009Article
- Inhibition of the c-Abl-TAp63 pathway protects mouse oocytes from chemotherapy-induced death.Nature medicine · 2009Article
- Cross-linking to an interrupted polypurine sequence with a platinum-modified triplex-forming oligonucleotide.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2009Article
- Development of an ultraperformance liquid chromatography/mass spectrometry method to quantify cisplatin 1,2 intrastrand guanine-guanine adducts.Chemical research in toxicology · 2009Article
- Adduct-specific monoclonal antibodies for the measurement of cisplatin-induced DNA lesions in individual cell nuclei.Nucleic acids research · 2006Article
- Hyperthermia enhances the cytotoxicity and platinum-DNA adduct formation of lobaplatin and oxaliplatin in cultured SW 1573 cells.Journal of cancer research and clinical oncology · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a sensitive 32P-postlabeling method for the detection of bifunctional intrastrand crosslinks d(Pt-GpG) and d(Pt-ApG) in DNA in vitro and in vivo. After enzymatic digestion of DNA the positively charged platinum adducts were purified from unplatinated products, using strong cation exchange chromatography. Subsequently the samples were deplatinated with cyanide, because platinated dinucleotides are very poor substrates for polynucleotide kinase. The excess of cyanide was removed using Sep-pak C18 cartridges, and the resulting dinucleoside monophosphates d(GpG) and d(ApG) were subsequently postlabelled. Analysis of the postlabelling mixture was performed by a combined TLC and HPLC-procedure. Good correlations with existing methods (AAS, immunocytochemistry and ELISA) were found in DNA samples treated in vitro and in vivo with cis- or carboplatin. The detection limit of the assay was 1 adduct/10(7) nucleotides in a 10 micrograms DNA sample.
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