ArticleJournal of muscle research and cell motility1995
The effect of 2,3-butanedione 2-monoxime (BDM) on ventricular trabeculae from the avian heart.
Article in Journal of muscle research and cell motility, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 19 citations in OpenAlex.
- Rats genetically selected for low and high aerobic capacity exhibit altered soleus muscle myofilament functions.American journal of physiology. Cell physiology · 2020Article
- Calcium buffering and excitation-contraction coupling in developing avian myocardium.Biophysical journal · 2004Article
- Kinetic effects of myosin regulatory light chain phosphorylation on skeletal muscle contraction.Biophysical journal · 2002Article
- Stability of the contractile assembly and Ca2+-activated tension in adenovirus infected adult cardiac myocytes.Molecular and cellular biochemistry · 1998Article
- Actin-titin interaction in cardiac myofibrils: probing a physiological role.Biophysical journal · 1997Article
- Limits of titin extension in single cardiac myofibrils.Journal of muscle research and cell motility · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
2,3-butanedione 2-monoxime (BDM, 3-30 mM) decreased twitch force of intact ventricular trabeculae isolated from 19-day embryonic chick hearts in a dose-dependent manner. The responses to BDM were rapid and reversible. In an attempt to determine the cellular basis for the inhibitory effect of BDM, experiments were carried out on skinned muscle fibres and isolated myocytes. In trabeculae skinned with Triton X-100, BDM depressed maximum calcium activated force (Fmax) with an IC50 of 14 mM. At 3 mM BDM, the proportional decrease in twitch force in intact tissue was similar to that of Fmax in skinned tissue. At higher BDM concentrations (10 and 30 mM), however, the proportional decrease in twitch force was greater than that of Fmax. BDM (up to 10 mM) had no effect on the normalized force-pCa relationship. In saponin-skinned preparations, BDM (3 and 30 mM) released calcium from the fully loaded sarcoplasmic reticulum to a slightly greater extent in the absence of calcium (pCa 8.5) than in the presence of a fixed level of free calcium (pCa 5.5). Whole cell patch clamping of freshly isolated chick myocytes demonstrated that BDM caused a dose-dependent decrease in the T- and L-type calcium current. Therefore, at low BDM concentrations (3 mM), the decrease in twitch force can be ascribed predominantly to depression of the contractile apparatus while, at higher concentrations of BDM, there is an additional inhibitory effect of BDM on excitation-contraction coupling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.