ArticleJournal of clinical microbiology1995
Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin.
Article in Journal of clinical microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 2 of them syntheses that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- In-house nucleic acid amplification tests for the detection of Mycobacterium tuberculosis in sputum specimens: meta-analysis and meta-regression.BMC microbiology · 2005Pooled it
- Assessment by meta-analysis of PCR for diagnosis of smear-negative pulmonary tuberculosis.Journal of clinical microbiology · 2003Pooled it
- Single-tube nested PCR in the diagnosis of tuberculosis.Journal of clinical pathology · 1996Trial
- Advances in TB testing.Advances in clinical chemistry · 2023Article
- Evaluation of the performance of an in-house duplex PCR assay targeting the IS6110 and rpoB genes for tuberculosis diagnosis in Cameroon.BMC infectious diseases · 2020Article
- Integrated Microfluidic Preconcentration and Nucleic Amplification System for Detection of Influenza A Virus H1N1 in Saliva.Micromachines · 2020Article
- Preanalytical conditions can interfere with M. tuberculosis detection by PCR in respiratory samples.Clinics (Sao Paulo, Brazil) · 2018Article
- Optimized Lysis-Extraction Method Combined WithFrontiers in microbiology · 2018Article
- A new assay based on terminal restriction fragment length polymorphism of homocitrate synthase gene fragments for Candida species identification.Journal of applied genetics · 2017Article
- Validation of a Multiplex Real-Time PCR Assay for Detection of Mycobacterium spp., Mycobacterium tuberculosis Complex, and Mycobacterium avium Complex Directly from Clinical Samples by Use of the BD Max Open System.Journal of clinical microbiology · 2016Article
- Evaluation of in-house PCR for diagnosis of smear-negative pulmonary tuberculosis in Kampala, Uganda.BMC research notes · 2012Article
- Rapid identification of mycobacteria and drug-resistant Mycobacterium tuberculosis by use of a single multiplex PCR and DNA sequencing.Journal of clinical microbiology · 2012Article
- Rapid DNA extraction for specific detection and quantitation of Mycobacterium tuberculosis DNA in sputum specimens using Taqman assays.Tuberculosis (Edinburgh, Scotland) · 2011Article
- Selective enrichment and detection of mycobacterial DNA in paucibacillary specimens.Journal of microbiological methods · 2006Article
- Novel multipurpose methodology for detection of mycobacteria in pulmonary and extrapulmonary specimens by smear microscopy, culture, and PCR.Journal of clinical microbiology · 2005Article
- Advances in Molecular Diagnosis of Tuberculosis.Medical journal, Armed Forces India · 2003Article
- Rapid diagnosis of extrapulmonary tuberculosis by PCR: impact of sample preparation and DNA extraction.Journal of clinical microbiology · 2003Article
- Quantitative PCR assay to measure Aspergillus fumigatus burden in a murine model of disseminated aspergillosis: demonstration of efficacy of caspofungin acetate.Antimicrobial agents and chemotherapy · 2001Article
- Simultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR.Journal of clinical microbiology · 1999Article
- Method for reduction of inhibition in a Mycobacterium tuberculosis-specific ligase chain reaction DNA amplification assay.Journal of clinical microbiology · 1998Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.