ArticleJournal of clinical microbiology1995
Improved detection of Candida albicans by PCR in blood of neutropenic mice with systemic candidiasis.
Article in Journal of clinical microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 87 citations in OpenAlex.
- Candida albicans white and opaque cells exhibit distinct spectra of organ colonization in mouse models of infection.PloS one · 2019Article
- Optimization of DNA extraction from human urinary samples for mycobiome community profiling.PloS one · 2019Article
- High-dose ascorbate with low-dose amphotericin B attenuates severity of disease in a model of the reappearance of candidemia during sepsis in the mouse.American journal of physiology. Regulatory, integrative and comparative physiology · 2015Article
- β-D-Glucan Assay in Diagnosis and Monitoring the Systemic Candidiasis in a Rat Model.Jundishapur journal of microbiology · 2014Article
- Effect of antifungal therapy timing on mortality in cancer patients with candidemia.Antimicrobial agents and chemotherapy · 2010Article
- Diagnosis of candidemia by polymerase chain reaction and blood culture: prospective study in a high-risk population and identification of variables associated with development of candidemia.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2005Article
- Clinical microbiology of bacterial and fungal sepsis in very-low-birth-weight infants.Clinical microbiology reviews · 2004Review
- Detection of fungemia by polymerase chain reaction in critically ill neonates and children.Journal of perinatology : official journal of the California Perinatal Association · 2003Article
- Primers ITS1, ITS2 and ITS4 detect the intraspecies variability in the internal transcribed spacers and 5.8S rRNA gene region in clinical isolates of fungi.Folia microbiologica · 2003Article
- Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification.Journal of clinical microbiology · 2002Article
- Current status of nonculture methods for diagnosis of invasive fungal infections.Clinical microbiology reviews · 2002Review
- Rapid identification of candida species by TaqMan PCR.Journal of clinical pathology · 2001Article
- Diagnosis and monitoring of murine histoplasmosis by a nested PCR assay.Journal of clinical microbiology · 2001Article
- Nucleic acid sequence-based amplification of Aspergillus RNA in blood samples.Journal of clinical microbiology · 2001Article
- Quantitative galactomannan detection is superior to PCR in diagnosing and monitoring invasive pulmonary aspergillosis in an experimental rat model.Journal of clinical microbiology · 2000Article
- Development of a species-specific PCR-restriction fragment length polymorphism analysis procedure for identification of Madurella mycetomatis.Journal of clinical microbiology · 1999Article
- PCR-restriction enzyme analysis for detection of Candida DNA in blood from febrile patients with hematological malignancies.Journal of clinical microbiology · 1999Article
- Identification of medically relevant Trichosporon species based on sequences of internal transcribed spacer regions and construction of a database for Trichosporon identification.Journal of clinical microbiology · 1999Article
- Serum is more suitable than whole blood for diagnosis of systemic candidiasis by nested PCR.Journal of clinical microbiology · 1999Article
- Identification of contaminating fungal DNA sequences in Zymolyase.Journal of clinical microbiology · 1999Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A PCR using primers aimed at the multicopy gene coding for the small subunit rRNA and resulting in the synthesis of a 180-bp fragment was evaluated for its use in diagnosing invasive candidiasis in comparison with blood culture. With the use of a C. albicans-specific probe, +/- 10 to 15 C. albicans cells are detected in 100 microliters of whole blood by Southern analysis. A DNase pretreatment was critical in the purification process of yeast DNA from whole blood. Omission of the DNase pretreatment decreased assay sensitivity 10-fold. PCR analysis of blood specimens collected from mice with invasive candidiasis is more sensitive than blood culture (100 versus 67%, respectively) at 72 h after intravenous (i.v.) inoculation with C. albicans. Furthermore, the intensity of the hybridization signals increased with the progression of infection. In contrast, multiple blood samples from gastrointestinally colonized mice were all negative by PCR, indicating that the PCR assay is also specific and may, therefore, make a positive contribution to the detection and follow-up of invasive candidiasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.