ArticleThe Journal of cell biology1995
Characterization of a minus end-directed kinesin-like motor protein from cultured mammalian cells.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 78 citations in OpenAlex.
- Kinesin-binding protein remodels the kinesin motor to prevent microtubule binding.Science advances · 2021Article
- Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.Molecular & cellular proteomics : MCP · 2013Article
- The molecular basis for kinesin functional specificity during mitosis.Cytoskeleton (Hoboken, N.J.) · 2013Review
- Mitotic chromosome biorientation in fission yeast is enhanced by dynein and a minus-end-directed, kinesin-like protein.Molecular biology of the cell · 2007Article
- Cell cycle-dependent dynamics and regulation of mitotic kinesins in Drosophila S2 cells.Molecular biology of the cell · 2005Article
- A minus-end-directed kinesin with plus-end tracking protein activity is involved in spindle morphogenesis.Molecular biology of the cell · 2005Article
- Mitotic regulation of protein 4.1R involves phosphorylation by cdc2 kinase.Molecular biology of the cell · 2005Article
- A mechanistic model for the organization of microtubule asters by motor and non-motor proteins in a mammalian mitotic extract.Molecular biology of the cell · 2004Article
- Characterization of Cep135, a novel coiled-coil centrosomal protein involved in microtubule organization in mammalian cells.The Journal of cell biology · 2002Article
- All kinesin superfamily protein, KIF, genes in mouse and human.Proceedings of the National Academy of Sciences of the United States of America · 2001Review
- Chromosome movement in mitosis requires microtubule anchorage at spindle poles.The Journal of cell biology · 2001Article
- Molecular cloning and functional analysis of mouse C-terminal kinesin motor KifC3.Molecular and cellular biology · 2001Article
- Kinesins in the Arabidopsis genome: a comparative analysis among eukaryotes.BMC genomics · 2001Article
- Article
- The kinesin-related protein, HSET, opposes the activity of Eg5 and cross-links microtubules in the mammalian mitotic spindle.The Journal of cell biology · 1999Article
- A nonerythroid isoform of protein 4.1R interacts with the nuclear mitotic apparatus (NuMA) protein.The Journal of cell biology · 1999Article
- Characterization of microtubule binding domains in the Arabidopsis kinesin-like calmodulin binding protein.The Plant cell · 1998Article
- Two-dimensional tracking of ncd motility by back focal plane interferometry.Biophysical journal · 1998Article
- XCTK2: a kinesin-related protein that promotes mitotic spindle assembly in Xenopus laevis egg extracts.The Journal of cell biology · 1997Article
- Fission yeast pkl1 is a kinesin-related protein involved in mitotic spindle function.Molecular biology of the cell · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Using the CHO2 monoclonal antibody raised against CHO spindles (Sellitto, C., M. Kimble, and R. Kuriyama. 1992. Cell Motil. Cytoskeleton. 22:7-24) we identified a 66-kD protein located at the interphase centrosome and mitotic spindle. Isolated cDNAs for the antigen encode a 622-amino acid polypeptide. Sequence analysis revealed the presence of 340-amino acid residues in the COOH terminus, which is homologous to the motor domain conserved among other members of the kinesin superfamily. The protein is composed of a central alpha-helical portion with globular domains at both NH2 and COOH termini, and the epitope to the monoclonal antibody resides in the central alpha-helical stalk. A series of deletion constructs were created for in vitro analysis of microtubule interactions. While the microtubule binding and bundling activities require both the presence of the COOH terminus and the alpha-helical domain, the NH2-terminal half of the antigen lacked the ability to interact with microtubules. The full-length as well as deleted proteins consisting of the COOH-terminal motor and the central alpha-helical stalk supported microtubule gliding, with velocity ranging from 1.0 to 8.4 microns/minute. The speed of microtubule movement decreased with decreasing lengths of the central stalk attached to the COOH-terminal motor. The microtubules moved with their plus end leading, indicating that the antigen is a minus end-directed motor. The CHO2 sequence shows 86% identify to HSET, a gene located at the centromeric end of the human MHC region in chromosome 6 (Ando, A., Y. Y. Kikuti, H. Kawata, N. Okamoto, T. Imai, T. Eki, K. Yokoyama, E. Soeda, T. Ikemura, K. Abe, and H. Inoko. 1994. Immunogenetics. 39:194-200), indicating that HSET might represent a human homologue of the CHO2 antigen.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.