ArticleClinical and experimental immunology1995
Synthesis and regulation of accessory/proinflammatory cytokines by intestinal epithelial cells.
Article in Clinical and experimental immunology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 72 citations in OpenAlex.
- Food Chemicals Disrupt Human Gut Microbiota Activity And Impact Intestinal Homeostasis As Revealed By In Vitro Systems.Scientific reports · 2018Article
- A Host-Produced Autoinducer-2 Mimic Activates Bacterial Quorum Sensing.Cell host & microbe · 2016Article
- Lloyd F. Mayer, MD: a remembrance.Mucosal immunology · 2014Article
- P2Y2 receptor transcription is increased by NF-kappa B and stimulates cyclooxygenase-2 expression and PGE2 released by intestinal epithelial cells.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Article
- Nucleotides enhance the secretion of interleukin 7 from primary-cultured murine intestinal epithelial cells.Cytotechnology · 2002Article
- Differential expression and upregulation of interleukin-1alpha, interleukin-1beta and interleukin-6 by freshly isolated human small intestinal epithelial cells.Mediators of inflammation · 2002Article
- Epithelial cells respond to proteolytic and non-proteolytic detachment by enhancing interleukin-6 responses.Immunology · 2002Article
- Characterization of conditions for the primary culture of human small intestinal epithelial cells.Clinical and experimental immunology · 2001Article
- Polarized secretion of CXC chemokines by human intestinal epithelial cells in response to Bacteroides fragilis enterotoxin: NF-kappa B plays a major role in the regulation of IL-8 expression.Clinical and experimental immunology · 2001Article
- Article
- Vectorial function of major histocompatibility complex class II in a human intestinal cell line.Immunology · 1999Article
- Factors affecting Caco-2 intestinal epithelial cell interleukin-6 secretion.In vitro cellular & developmental biology. Animal · 1998Article
- Differential early interactions between Salmonella enterica serovar Typhi and two other pathogenic Salmonella serovars with intestinal epithelial cells.Infection and immunity · 1998Article
- Regulation of mucosal immune responses: distinct antigens and antigen presenting cells.Journal of clinical immunology · 1997Review
- Clostridium perfringens enterotoxin lacks superantigenic activity but induces an interleukin-6 response from human peripheral blood mononuclear cells.Infection and immunity · 1997Article
- Immunopathology of human inflammatory bowel disease.Springer seminars in immunopathology · 1997Review
- The intestinal epithelial cell: immunological aspects.Springer seminars in immunopathology · 1997Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Intestinal epithelial cells (IEC) have been shown to act as antigen-presenting cells (APC) in vitro and may have this capacity in vivo. In order to determine whether IEC, like other APC, are able to produce accessory cytokines which may play a role in T cell activation, we assessed the accessory cytokine profile of IEC constitutively or after stimulation. We measured expression, production and regulation of accessory cytokines (IL-1 beta, IL-6, tumour necrosis factor-alpha (TNF-alpha), transforming growth factor-beta (TGF-beta) by the presence of mRNA as well as secreted protein. Freshly isolated IEC from surgical specimens were cultured in the presence or absence of lipopolysaccharide (LPS), interferon-gamma (IFN-gamma), IL-1 beta or TNF-alpha. mRNA was assessed by a specific RNAse protection assay which controlled for contaminating cell populations while protein secretion was measured by ELISA (IL-1) or bioassay (TNF and IL-6). Neither IL-1 beta nor TNF-alpha were detectable in cultured IEC supernatants, supporting the lack of macrophage contamination. All IEC spontaneously secreted IL-6 at levels comparable to those of macrophages. IEC IL-6 mRNA also increased approximately 200-fold during the first 24 h of culture. LPS, IFN-gamma or TNF-alpha had no effect on spontaneous IL-6 production, and neither resulted in the secretion of IL-1 beta or TNF-alpha. However, IL-1 beta up-regulated IL-6 synthesis by 6-7-fold. IEC express a profile of cytokine mRNAs distinct from conventional APC (low level constitutive IL-6 expression but no detectable IL-1 beta, TGF-beta or TNF-alpha), adding to their uniqueness as APC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.