ArticleThe Biochemical journal1995
Stimulation of hyaluronan biosynthesis by platelet-derived growth factor-BB and transforming growth factor-beta 1 involves activation of protein kinase C.
Article in The Biochemical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 134 citations in OpenAlex.
- Cell-Free Blood Cell Secretome (BCS) Counteracts Skin Aging: Multi-Center Prospective Regenerative Aesthetic Medicine Study Using Exokine®.Clinical, cosmetic and investigational dermatology · 2022Trial
- Effects of Hyaluronan on Breast Cancer Aggressiveness.Cancers · 2023Review
- Inheritance of Monogenic Hereditary Skin Disease and Related Canine Breeds.Veterinary sciences · 2022Review
- Dendritic cell entry to lymphatic capillaries is orchestrated by CD44 and the hyaluronan glycocalyx.Life science alliance · 2021Article
- The deubiquitinating enzymes USP4 and USP17 target hyaluronan synthase 2 and differentially affect its function.Oncogenesis · 2017Article
- Article
- Biology and biotechnology of hyaluronan.Glycoconjugate journal · 2015Review
- Increased hyaluronan levels in HABP1/p32/gC1qR overexpressing HepG2 cells inhibit autophagic vacuolation regulating tumor potency.PloS one · 2014Article
- Hyaluronan, a crucial regulator of inflammation.Frontiers in immunology · 2014Review
- Hyaluronan synthase 2 (HAS2) promotes breast cancer cell invasion by suppression of tissue metalloproteinase inhibitor 1 (TIMP-1).The Journal of biological chemistry · 2011Article
- Hyaluronan production and hyaluronan synthase expression in three human conjunctiva-derived cell strains.Japanese journal of ophthalmology · 2011Article
- The activity of hyaluronan synthase 2 is regulated by dimerization and ubiquitination.The Journal of biological chemistry · 2010Article
- Cloning and characterization of cell strains derived from human corneal stroma and sclera.Japanese journal of ophthalmology · 2010Article
- Modulation of hyaluronan synthase activity in cellular membrane fractions.The Journal of biological chemistry · 2009Article
- Signal pathways regulating hyaluronan secretion into static and cycled synovial joints of rabbits.The Journal of physiology · 2009Article
- Enhanced flexor tendon healing through controlled delivery of PDGF-BB.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2009Article
- High levels of hyaluronan in idiopathic pulmonary arterial hypertension.American journal of physiology. Lung cellular and molecular physiology · 2008Article
- Growth factor regulation of hyaluronan synthesis and degradation in human dermal fibroblasts: importance of hyaluronan for the mitogenic response of PDGF-BB.The Biochemical journal · 2007Article
- Recombinant human hyaluronan synthase 3 is phosphorylated in mammalian cells.The Biochemical journal · 2006Article
- Bench-to-bedside review: the role of glycosaminoglycans in respiratory disease.Critical care (London, England) · 2006Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intracellular signal transduction pathways that mediate the stimulatory effects of platelet-derived growth factor (PDGF)-BB and transforming growth factor (TGF)-beta on hyaluronan biosynthesis in human fibroblasts were investigated. The stimulatory effects of both PDGF-BB and TGF-beta 1 were dependent on protein kinase C (PKC), since the PKC inhibitor calphostin C inhibited the stimulation by the growth factors. Direct activation of PKC by phorbol 12-myristate 13-acetate (PMA) also stimulated hyaluronan production, and the combination of either PDGF-BB or TGF-beta 1 and PMA gave an increased effect. One possible mechanism for activation of PKC is via induction of phospholipase C (PLC) activity; U-17322, an inhibitor of PLC-gamma, was found to inhibit partially PDGF-BB-stimulated hyaluronan synthesis. PDGF-BB is known to activate PLC-gamma through tyrosine phosphorylation; however, a PDGF beta-receptor mutant unable to interact with and activate PLC-gamma was still able to mediate induction of hyaluronan biosynthesis, indicating that PDGF-mediated stimulation is not entirely dependent on PLC-gamma. The stimulations by PDGF-BB and TGF-beta 1 were partly dependent on protein synthesis, since parts of the effects were inhibited by cycloheximide; in contrast, the effects mediated by PMA were not. Our results indicate that PKC is involved in the transduction of the effects of growth factors on hyaluronan biosynthesis, and that the effects involve direct or indirect activation of existing hyaluronan synthetase molecules, as well as induction of new enzyme molecules.
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