ArticleMolecular and cellular biology1995
The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Protocol for analyzing protein interactomes under starvation in fission yeast using in vivo cross-linking.STAR protocols · 2026Article
- Article
- Nrm1 is a bistable switch connecting cell cycle progression to transcriptional control.EMBO reports · 2025Article
- Article
- SWI/SNF and RSC remodeler complexes bind to MBF-dependent genes.Cell cycle (Georgetown, Tex.) · 2021Article
- Recent insights into mechanisms preventing ectopic centromere formation.Open biology · 2021Review
- Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave.Nucleic acids research · 2019Article
- Down-regulation of Cdk1 activity in G1 coordinates the G1/S gene expression programme with genome replication.Current genetics · 2019Review
- Cell Cycle-Regulated Transcription of CENP-A by the MBF Complex Ensures Optimal Level of CENP-A for Centromere Formation.Genetics · 2019Article
- Lack of a peroxiredoxin suppresses the lethality of cells devoid of electron donors by channelling electrons to oxidized ribonucleotide reductase.PLoS genetics · 2017Article
- The Fission Yeast Pre-mRNA-processing Factor 18 (prp18+) Has Intron-specific Splicing Functions with Links to G1-S Cell Cycle Progression.The Journal of biological chemistry · 2016Article
- A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave.Cell cycle (Georgetown, Tex.) · 2016Article
- Structural basis of DNA recognition by PCG2 reveals a novel DNA binding mode for winged helix-turn-helix domains.Nucleic acids research · 2015Article
- The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor.Molecular biology of the cell · 2013Article
- G1/S transcription factor orthologues Swi4p and Swi6p are important but not essential for cell proliferation and influence hyphal development in the fungal pathogen Candida albicans.Eukaryotic cell · 2011Article
- Role for cohesin in the formation of a heterochromatic domain at fission yeast subtelomeres.Molecular and cellular biology · 2011Article
- Phosphorylation of the MBF repressor Yox1p by the DNA replication checkpoint keeps the G1/S cell-cycle transcriptional program active.PloS one · 2011Article
- Cell-cycle regulation of cohesin stability along fission yeast chromosomes.The EMBO journal · 2008Article
- The cell cycle-regulated genes of Schizosaccharomyces pombe.PLoS biology · 2005Article
- Cell cycle-regulated transcription in fission yeast: Cdc10-Res protein interactions during the cell cycle and domains required for regulated transcription.Molecular biology of the cell · 1999Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Schizosaccharomyces pombe, MBF is a DNA-binding complex suspected to activate the transcription of genes necessary for entry into S phase. The MBF complex contains both p85cdc10 and p72res1/sct1. To obtain a better understanding of how the MBF complex regulates gene expression at the G1/S transition, we have performed a genetic analysis of p72res1. We determined that p72res1 can bind specifically to the cdc22 promoter, when analyzed by gel mobility shift assay, and that the N-terminal 157 amino acids of p72res1 are sufficient for this specific binding. When overexpressed in vivo, a fragment of p72res1 containing this DNA-binding domain could rescue a strain carrying a temperature-sensitive cdc10 allele at the restrictive temperature as well as a strain with a cdc10 null allele. We also determined that the C-terminal region of p72res1 is necessary and sufficient for binding to p85cdc10. Overexpression of the cdc10-binding domain of p72res1 leads to a G1 arrest with a cdc phenotype and a decrease on MBF activity. Overexpression of full-length p72res1 also leads to a growth arrest that can be rescued by overexpression of p85cdc10. These results imply that the MBF activity in vivo is dependent on the interaction of p85cdc10 with p72res1.
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