ArticleMolecular and cellular biology1995
Mapping of replication initiation sites in human ribosomal DNA by nascent-strand abundance analysis.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 68 citations in OpenAlex.
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- Allele-specific genome-wide profiling in human primary erythroblasts reveal replication program organization.PLoS genetics · 2014Article
- A comprehensive genome-wide map of autonomously replicating sequences in a naive genome.PLoS genetics · 2010Article
- DNA replication origin plasticity and perturbed fork progression in human inverted repeats.Molecular and cellular biology · 2005Article
- Coordination of replication and transcription along a Drosophila chromosome.Genes & development · 2004Article
- Site-specific and temporally controlled initiation of DNA replication in a human cell-free system.Nucleic acids research · 2002Article
- Replication initiation patterns in the beta-globin loci of totipotent and differentiated murine cells: evidence for multiple initiation regions.Molecular and cellular biology · 2002Article
- Enhanced flexibility and aphidicolin-induced DNA breaks near mammalian replication origins: implications for replicon mapping and chromosome fragility.Nucleic acids research · 2000Article
- Conversion of topoisomerase I cleavage complexes on the leading strand of ribosomal DNA into 5'-phosphorylated DNA double-strand breaks by replication runoff.Molecular and cellular biology · 2000Article
- An origin of bidirectional DNA replication is located within a CpG island at the 3" end of the chicken lysozyme gene.Nucleic acids research · 1999Article
- Identification of primary initiation sites for DNA replication in the hamster dihydrofolate reductase gene initiation zone.Molecular and cellular biology · 1998Article
- oriGNAI3: a narrow zone of preferential replication initiation in mammalian cells identified by 2D gel and competitive PCR replicon mapping techniques.Nucleic acids research · 1998Article
- Replication initiation and elongation fork rates within a differentially expressed human multicopy locus in early S phase.Nucleic acids research · 1997Article
- Precise switching of DNA replication timing in the GC content transition area in the human major histocompatibility complex.Molecular and cellular biology · 1997Article
- Relative distribution of rDNA and proteins of the RNA polymerase I transcription machinery at chromosomal NORs.Chromosoma · 1997Article
- Active mammalian replication origins are associated with a high-density cluster of mCpG dinucleotides.Molecular and cellular biology · 1997Article
- Chromosome architecture can dictate site-specific initiation of DNA replication in Xenopus egg extracts.The Journal of cell biology · 1996Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
New techniques for mapping mammalian DNA replication origins are needed. We have modified the existing nascent-strand size analysis technique (L. Vassilev and E.M. Johnson, Nucleic Acids Res. 17:7693-7705, 1989) to provide an independent means of studying replication initiation sites. We call the new method nascent-strand abundance analysis. We confirmed the validity of this method with replicating simian virus 40 DNA as a model. We then applied nascent-strand abundance and nascent-strand size analyses to mapping of initiation sites in human (HeLa) ribosomal DNA (rDNA), a region previously examined exclusively by two-dimensional gel electrophoresis methods (R.D. Little, T.H.K. Platt, and C.L. Schildkraut, Mol. Cell. Biol. 13:6600-6613, 1993). Our results partly confirm those obtained by two-dimensional gel electrophoresis techniques. Both studies suggest that replication initiates at relatively high frequency a few kilobase pairs upstream of the transcribed region and that many additional low-frequency initiation sites are distributed through most of the remainder of the ribosomal DNA repeat unit.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.