ArticleThe EMBO journal1995
Cloning of a cDNA for lamina-associated polypeptide 2 (LAP2) and identification of regions that specify targeting to the nuclear envelope.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 179 citations in OpenAlex.
- Lamin B receptor upregulation in metastatic melanoma causes nuclear envelope fragility in confined migration during cancer invasion.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cell Senescence and Central Regulators of Immune Response.International journal of molecular sciences · 2022Review
- pUL21 regulation of pUs3 kinase activity influences the nature of nuclear envelope deformation by the HSV-2 nuclear egress complex.PLoS pathogens · 2021Article
- Histone methyltransferase activity programs nuclear peripheral genome positioning.Developmental biology · 2020Article
- Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane.The Journal of biological chemistry · 2019Article
- Prognostic significance and biological function of Lamina-associated polypeptide 2 in non-small-cell lung cancer.OncoTargets and therapy · 2019Article
- Mechanisms of allelic and clinical heterogeneity of lamin A/C phenotypes.Physiological genomics · 2018Review
- Article
- Hutterite-type cataract maps to chromosome 6p21.32-p21.31, cosegregates with a homozygous mutation in LEMD2, and is associated with sudden cardiac death.Molecular genetics & genomic medicine · 2016Article
- Lamina-associated polypeptide (LAP)2α and nucleoplasmic lamins in adult stem cell regulation and disease.Seminars in cell & developmental biology · 2014Review
- LAP2 is widely overexpressed in diverse digestive tract cancers and regulates motility of cancer cells.PloS one · 2012Article
- Evolvement of LEM proteins as chromatin tethers at the nuclear periphery.Biochemical Society transactions · 2011Review
- Article
- Presenilin-1 processing of ErbB4 in fetal type II cells is necessary for control of fetal lung maturation.Biochimica et biophysica acta · 2011Article
- Lamin-binding Proteins.Cold Spring Harbor perspectives in biology · 2010Review
- Membrane-anchored growth factor, HB-EGF, on the cell surface targeted to the inner nuclear membrane.The Journal of cell biology · 2008Article
- TPX2 is required for postmitotic nuclear assembly in cell-free Xenopus laevis egg extracts.The Journal of cell biology · 2006Article
- Dependence of diffusional mobility of integral inner nuclear membrane proteins on A-type lamins.Biochemistry · 2006Article
- Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore.The Journal of cell biology · 2004Article
- LAP2alpha and BAF collaborate to organize the Moloney murine leukemia virus preintegration complex.The EMBO journal · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lamina-associated polypeptide 2 (LAP2) is an integral membrane protein of the inner nuclear membrane, which binds directly to both lamin B1 and chromosomes in a mitotic phosphorylation-regulated manner. The biochemical and physiological properties of LAP2 suggest an important role in nuclear envelope re-assembly at the end of mitosis and/or anchoring of the nuclear lamina and interphase chromosomes to the nuclear envelope. We describe the cDNA cloning of LAP2 and characterization of its membrane topology and targeting to the nuclear envelope. The LAP2 cDNA sequence predicts a protein of 452 amino acids, containing a large hydrophilic domain with several potential cdc2 kinase phosphorylation sites and a single putative membrane-spanning sequence at residues 410-433. Immunogold localization of an LAP2 epitope in isolated nuclear envelopes indicates that the large amino-terminal hydrophilic domain (residues 1-409) is exposed to the nucleoplasm. By expressing deletion mutants of LAP2 in cultured cells, we have identified multiple regions in its nucleoplasmic domain that promote localization at the nuclear envelope. These data suggest that targeting of LAP2 to the nuclear envelope is mediated by cooperative interactions with multiple binding sites at the inner nuclear membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.