ArticleThe Biochemical journal1995
Molecular cloning, purification and in situ localization of human colon kallikrein.
Article in The Biochemical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed, 34 citations in OpenAlex.
- Immunolocalization and expression of kallistatin and tissue kallikrein in human inflammatory bowel disease.Digestive diseases and sciences · 2003Article
- Tissue kallikrein and bradykinin B2 receptor in human uterus in luteal phase and in early and late gestation.Endocrine · 2001Article
- A synthetic tissue kallikrein inhibitor suppresses cancer cell invasiveness.The American journal of pathology · 2001Article
- Tissue kallikrein in human placenta in early and late gestation.Endocrine · 2001Article
- Crystal structure of recombinant human tissue kallikrein at 2.0 A resolution.Protein science : a publication of the Protein Society · 1998Article
- Recombinant hirustasin: production in yeast, crystallization, and interaction with serine proteases.Protein science : a publication of the Protein Society · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have cloned and characterized a full-length cDNA encoding tissue kallikrein from a human colon carcinoma cell line (T84). The nucleic acid sequence of the colon kallikrein cDNA is identical to that of renal/pancreatic or tissue kallikrein cDNA. Reverse-transcription PCR followed by Southern-blot analysis using specific oligonucleotide probes showed expression of tissue kallikrein in human colon, pancreas and kidney. Tissue kallikrein mRNA was localized in glandular epithelial cells (goblet cells) in colon by in situ hybridization histochemistry. Human colon kallikrein was purified to apparent homogeneity by DEAE-Sepharose Cl-6B, aprotinin-affinity, and HQ/M perfusion chromatography. The purified colon kallikrein migrated as a broad, 40-45 kDa band in SDS/PAGE and was recognized by antibodies to human tissue kallikrein. The linear displacement curves for the colon kallikrein in an RIA were parallel with the human tissue kallikrein standard curve, indicating their immunological identity. The N-terminal sequence of the purified colon kallikrein matches completely with that of purified urinary or tissue kallikrein. These results indicate that human colon kallikrein is transcribed from the tissue kallikrein gene.
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Registered trials
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