ArticleNucleic acids research1995
Single base discrimination for ribonuclease H-dependent antisense effects within intact human leukaemia cells.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 85 citations in OpenAlex.
- Allele-Selective Thiomorpholino Antisense Oligonucleotides as a Therapeutic Approach for Fused-in-Sarcoma Amyotrophic Lateral Sclerosis.International journal of molecular sciences · 2024Article
- Influence of mismatched and bulged nucleotides on SNP-preferential RNase H cleavage of RNA-antisense gapmer heteroduplexes.Scientific reports · 2017Article
- Molecular beacons: powerful tools for imaging RNA in living cells.Journal of nucleic acids · 2011Article
- NANOSTRUCTURED PROBES FOR IN VIVO GENE DETECTION.Nanomedicine : design and applications of magnetic nanomaterials, nanosensors, and nanosystems · 2010Article
- Gene silencing by synthetic U1 adaptors.Nature biotechnology · 2009Article
- Fluorescent probes for live-cell RNA detection.Annual review of biomedical engineering · 2009Review
- Cellular antisense activity of peptide nucleic acid (PNAs) targeted to HIV-1 polypurine tract (PPT) containing RNA.Nucleic acids research · 2007Article
- Cross-clade inhibition of HIV-1 replication and cytopathology by using RNase P-associated external guide sequences.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Transient suppression of transgene expression by means of antisense oligonucleotides: a method for the production of toxin-transducing recombinant viruses.Gene therapy · 2002Article
- Long-term RNase P-mediated inhibition of HIV-1 replication and pathogenesis.Gene therapy · 2001Article
- Reduction of target gene expression by a modified U1 snRNA.Molecular and cellular biology · 2001Article
- Antisense therapeutics in chronic myeloid leukaemia: the promise, the progress and the problems.Leukemia · 2000Review
- Keeping the biotechnology of antisense in context.Nature biotechnology · 1999Article
- Cloning of the cDNA encoding the large subunit of human RNase HI, a homologue of the prokaryotic RNase HII.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Real time detection of DNA.RNA hybridization in living cells.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Use of ribozymes and antisense oligodeoxynucleotides to investigate mechanisms of drug resistance.Cytotechnology · 1998Article
- Selecting optimal oligonucleotide composition for maximal antisense effect following streptolysin O-mediated delivery into human leukaemia cells.Nucleic acids research · 1998Article
- Antisense inhibition of virus infections.Advances in pharmacology (San Diego, Calif.) · 1997Review
- Antisense oligonucleotide containing an internal, non-nucleotide-based linker promote site-specific cleavage of RNA.Nucleic acids research · 1996Article
- Rational design of point mutation-selective antisense DNA targeted to codon 12 of Ha-ras mRNA in human cells.Nucleic acids research · 1995Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously demonstrated, in vitro, that phosphodiester and phosphorothioate antisense oligodeoxynucleotides could direct ribonuclease H to cleave non-target RNA sites and that chimeric methylphosphonodiester/phosphodiester analogue structures were substantially more specific. In this report we show that such chimeric molecules can promote point mutation-specific scission of target mRNA by both Escherichia coli and human RNases H in vitro. Intact human leukaemia cells 'biochemically microinjected' with antisense effectors demonstrated efficient suppression of target mRNA expression. It was noted that the chimeric methylphosphonodiester/phosphodiester structures showed single base discrimination, whereas neither the phosphodiester nor phosphorothioate compounds were as stringent. Finally, we show that the antisense effects obtained in intact cells were due to endogenous RNase H activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.