ArticleThe Journal of cell biology1995
Reassembly of Golgi stacks from mitotic Golgi fragments in a cell-free system.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed.
- RNA scaffolds the Golgi ribbon by forming condensates with GM130.Nature cell biology · 2024Article
- Theoretical approaches for understanding the self-organized formation of the Golgi apparatus.Development, growth & differentiation · 2023Review
- Quantitative Proteomics Analysis of Purified Rat Liver Golgi.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Common Assays in Mammalian Golgi Studies.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Retro-2 alters Golgi structure.Scientific reports · 2022Article
- Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.The Journal of cell biology · 2021Article
- The function of GORASPs in Golgi apparatus organization in vivo.The Journal of cell biology · 2020Article
- Golgin45-Syntaxin5 Interaction Contributes to Structural Integrity of the Golgi Stack.Scientific reports · 2019Article
- GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion.Developmental cell · 2018Article
- Golgi apparatus self-organizes into the characteristic shape via postmitotic reassembly dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Structural Basis for the Interaction between Golgi Reassembly-stacking Protein GRASP55 and Golgin45.The Journal of biological chemistry · 2017Article
- Golgi structure formation, function, and post-translational modifications in mammalian cells.F1000Research · 2017Review
- GRASP: A Multitasking Tether.Frontiers in cell and developmental biology · 2016Review
- Structural basis for the interaction between the Golgi reassembly-stacking protein GRASP65 and the Golgi matrix protein GM130.The Journal of biological chemistry · 2015Article
- Golgi isolation.Cold Spring Harbor protocols · 2015Article
- Membrane adhesion dictates Golgi stacking and cisternal morphology.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Phosphorylation regulates VCIP135 function in Golgi membrane fusion during the cell cycle.Journal of cell science · 2014Article
- Cell-free reconstitution of vacuole membrane fragmentation reveals regulation of vacuole size and number by TORC1.Molecular biology of the cell · 2012Article
- Quantitative analysis of liver Golgi proteome in the cell cycle.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Clathrin is required for postmitotic Golgi reassembly.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rat liver Golgi stacks were incubated with mitotic cytosol for 30 min at 37 degrees C to generate mitotic Golgi fragments comprising vesicles, tubules, and cisternal remnants. These were isolated and further incubated with rat liver cytosol for 60 min. The earliest intermediate observed by electron microscopy was a single, curved cisterna with tubular networks fused to the cisternal rims. Elongation of this cisterna was accompanied by stacking and further growth at the cisternal rims. Stacks also fused laterally so that the typical end product was a highly curved stack of 2-3 cisternae mostly enclosing an electron-lucent space. Reassembly occurred in the presence of nocodazole or cytochalasin B but not at 4 degrees C or in the absence of energy supplied in the form of ATP and GTP. Pretreatment of the mitotic fragments and cytosol with N-ethylmaleimide (NEM) also prevented reassembly. GTP gamma S and A1F prevented reassembly when added during fragmentation but not when added to the reassembly mixture. In fact, GTP gamma S stimulated reassembly such that all cisternae were stacked at the end of the incubation and comprised 40% of the total membrane. In contrast, microcystin inhibited stacking so that only single cisternae accumulated. Together these results provide a detailed picture of the reassembly process and open up the study of the architecture of the Golgi apparatus to a combined morphological and biochemical analysis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.