ArticleThe Journal of cell biology1995
Reconstitution of vesiculated Golgi membranes into stacks of cisternae: requirement of NSF in stack formation.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 42 citations in OpenAlex.
- Signaling at the Golgi during mitosis.Methods in cell biology · 2013Review
- Phospholipase C beta3 is a key component in the Gbetagamma/PKCeta/PKD-mediated regulation of trans-Golgi network to plasma membrane transport.The Biochemical journal · 2007Article
- Membrane tubule-mediated reassembly and maintenance of the Golgi complex is disrupted by phospholipase A2 antagonists.Molecular biology of the cell · 1999Article
- Homotypic fusion of immature secretory granules during maturation in a cell-free assay.The Journal of cell biology · 1998Article
- In vitro reconstitution of microtubule plus end-directed, GTPgammaS-sensitive motility of Golgi membranes.Molecular biology of the cell · 1998Article
- Cruising along microtubule highways: how membranes move through the secretory pathway.The Journal of cell biology · 1998Review
- The Golgi and endoplasmic reticulum remain independent during mitosis in HeLa cells.Molecular biology of the cell · 1998Article
- Role of NAD+ and ADP-ribosylation in the maintenance of the Golgi structure.The Journal of cell biology · 1997Article
- Dissociation of coatomer from membranes is required for brefeldin A-induced transfer of Golgi enzymes to the endoplasmic reticulum.The Journal of cell biology · 1997Article
- Retrograde transport of mutant ricin to the endoplasmic reticulum with subsequent translocation to cytosol.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Homotypic vacuole fusion requires Sec17p (yeast alpha-SNAP) and Sec18p (yeast NSF).The EMBO journal · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have developed an in vitro system to study the biochemical events in the fusion of ilimaquinone (IQ) induced vesiculated Golgi membranes (VGMs) into stacks of cisternae. The Golgi complex in intact normal rat kidney cells (NRK) is vesiculated by treatment with IQ. The cells are washed to remove the drug and then permeabilized by a rapid freeze-thaw procedure. VGMs of 60 nm average diameter assemble into stacks of Golgi cisternae by a process that is temperature dependent, requires ATP and a high speed supernatant from cell extract (cytosol), as revealed by immunofluorescence and electron microscopy. The newly assembled stacks are functionally active in vesicular protein transport and contain processing enzymes that carry out Golgi specific modifications of glycoproteins. The fusion of VGMs requires NSF, a protein known to promote fusion of transport vesicles with the target membrane in the exocytic and endocytic pathways. Immunoelectron microscopy using Golgi specific anti-mannosidase II antibody reveals that VGMs undergo sequential changes in their morphology, whereby they first fuse to form larger vesicles of 200-300-nm average diameter which subsequently extend into tubular elements and finally assemble into stacks of cisternae.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.