ArticleInfection and immunity1995
A recombinant Leishmania chagasi antigen that stimulates cellular immune responses in infected mice.
Article in Infection and immunity, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 67 citations in OpenAlex.
- Vaccination in Leishmaniasis: A Review Article.Iranian biomedical journal · 2022Article
- Structure and dynamics studies of sterol 24-C-methyltransferase with mechanism based inactivators for the disruption of ergosterol biosynthesis.Molecular biology reports · 2014Article
- Immune responses of Iranian patients with visceral leishmaniasis and recovered individuals to LCR1 of Leishmania infantum.Clinical and vaccine immunology : CVI · 2014Article
- Visceral Leishmaniasis: Advancements in Vaccine Development via Classical and Molecular Approaches.Frontiers in immunology · 2014Review
- Development of Vaccines against Visceral Leishmaniasis.Journal of tropical medicine · 2012Article
- Leishmania infantum sterol 24-c-methyltransferase formulated with MPL-SE induces cross-protection against L. major infection.Vaccine · 2009Article
- A killed Leishmania vaccine with sand fly saliva extract and saponin adjuvant displays immunogenicity in dogs.Vaccine · 2008Article
- Article
- Protective immunization against visceral leishmaniasis using Leishmania sterol 24-c-methyltransferase formulated in adjuvant.Vaccine · 2007Article
- Leishmania chagasi T-cell antigens identified through a double library screen.Infection and immunity · 2006Article
- Evolutionary relationships and protein domain architecture in an expanded calpain superfamily in kinetoplastid parasites.Journal of molecular evolution · 2005Article
- Characterization of Leishmania donovani antigens encapsulated in liposomes that induce protective immunity in BALB/c mice.Infection and immunity · 2002Article
- Leishmania donovani p36(LACK) DNA vaccine is highly immunogenic but not protective against experimental visceral leishmaniasis.Infection and immunity · 2001Article
- A novel Leishmania infantum recombinant antigen which elicits interleukin 10 production by peripheral blood mononuclear cells of patients with visceral leishmaniasis.Infection and immunity · 2000Article
- Isolation of T-cell antigens by using a recombinant protein library and its application to the identification of novel vaccine candidates against schistosomiasis.Infection and immunity · 1999Article
- Human and murine immune responses to a novel Leishmania major recombinant protein encoded by members of a multicopy gene family.Infection and immunity · 1998Article
- Biochemical analysis and immunogenicity of Leishmania major amastigote fractions in cutaneous leishmaniasis.Clinical and experimental immunology · 1997Article
- Adjuvanticity and protective immunity elicited by Leishmania donovani antigens encapsulated in positively charged liposomes.Infection and immunity · 1997Article
- Characterization of an antigen from Leishmania amazonensis amastigotes able to elicit protective responses in a murine model.Infection and immunity · 1997Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Cellular immune mechanisms resulting in gamma interferon production are critical for protection against visceral leishmaniasis. Antigens stimulating T-cell responses are likely present in the intracellular amastigote form of the parasite, since this is the form found in a mammalian host. To identify T-cell antigens of Leishmania chagasi, the parasite causing South American visceral leishmaniasis, we used a double antibody-T-cell technique to screen an amastigote cDNA library. One cDNA selected (Lcr1) encodes an antigen that stimulated proliferation of splenic T lymphocytes from infected mice that were either resistant (C3H.HeJ) or susceptible (BALB/c) to L. chagasi infection. The Lcr1 cDNA contains four highly divergent 201-bp repeats homologous to the 204-bp repeat of a Trypanosoma cruzi flagellar antigen gene. Results are consistent with a single copy of the Lcr1 gene producing an mRNA of > 10 kb and a protein of > 200 kDa. Recombinant Lcr1, cloned adjacent to polyhistidine and purified on a nickel affinity column, stimulated gamma interferon but not interleukin-4 (IL-4), IL-5, or IL-10 secretion by T-cell-enriched splenocytes from either susceptible or resistant mice during L. chagasi infection. Immunization with Lcr1 partially protected BALB/c mice against challenge with L. chagasi, indicating the utility of the double screening approach in selecting relevant T-cell antigens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.