ArticleThe EMBO journal1995
A focal adhesion factor directly linking intracellularly motile Listeria monocytogenes and Listeria ivanovii to the actin-based cytoskeleton of mammalian cells.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
82 citing papers in PubMed, 269 citations in OpenAlex.
- Pushing boundaries: mechanisms enabling bacterial pathogens to spread between cells.Infection and immunity · 2024Review
- Review
- Phosphoproteomic Analysis ofFrontiers in cellular and infection microbiology · 2021Article
- Capping protein is dispensable for polarized actin network growth and actin-based motility.The Journal of biological chemistry · 2020Article
- Transfer of HTLV-1 p8 and Gag to target T-cells depends on VASP, a novel interaction partner of p8.PLoS pathogens · 2020Article
- Recent advances in understandingF1000Research · 2017Review
- Actin-based motility of bacterial pathogens: mechanistic diversity and its impact on virulence.Pathogens and disease · 2016Article
- Quantitative Analysis of Filament Branch Orientation in Listeria Actin Comet Tails.Biophysical journal · 2016Article
- Vasodilator-stimulated phosphoprotein restricts cell-to-cell spread of Shigella flexneri at the cell periphery.Microbiology (Reading, England) · 2015Article
- Reconstitution of actin-based motility by vasodilator-stimulated phosphoprotein (VASP) depends on the recruitment of F-actin seeds from the solution produced by cofilin.The Journal of biological chemistry · 2014Article
- Scaling of immune responses against intracellular bacterial infection.The EMBO journal · 2014Review
- Review
- ActA promotes Listeria monocytogenes aggregation, intestinal colonization and carriage.PLoS pathogens · 2013Article
- RIG-I detects infection with live Listeria by sensing secreted bacterial nucleic acids.The EMBO journal · 2012Article
- Incidence of Listeria spp. in Dairy Cows Feed and Raw Milk in Latvia.ISRN veterinary science · 2012Article
- Association of ActA to peptidoglycan revealed by cell wall proteomics of intracellular Listeria monocytogenes.The Journal of biological chemistry · 2011Article
- The role of C. elegans Ena/VASP homolog UNC-34 in neuronal polarity and motility.Developmental biology · 2010Article
- Poor invasion of trophoblastic cells but normal plaque formation in fibroblastic cells despite actA deletion in a group of Listeria monocytogenes strains persisting in some food processing environments.Applied and environmental microbiology · 2010Article
- Galleria mellonella as a model system for studying Listeria pathogenesis.Applied and environmental microbiology · 2010Article
- The integrin adhesion complex changes its composition and function during morphogenesis of an epithelium.Journal of cell science · 2009Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The surface-bound ActA polypeptide of the intracellular bacterial pathogen Listeria monocytogenes is the sole listerial factor needed for recruitment of host actin filaments by intracellularly motile bacteria. Here we report that following Listeria infection the host vasodilator-stimulated phosphoprotein (VASP), a microfilament- and focal adhesion-associated substrate of both the cAMP- and cGMP-dependent protein kinases, accumulates on the surface of intracytoplasmic bacteria prior to the detection of F-actin 'clouds'. VASP remains associated with the surface of highly motile bacteria, where it is polarly located, juxtaposed between one extremity of the bacterial surface and the front of the actin comet tail. Since actin filament polymerization occurs only at the very front of the tail, VASP exhibits properties of a host protein required to promote actin polymerization. Purified VASP binds directly to the ActA polypeptide in vitro. A ligand-overlay blot using purified radiolabelled VASP enabled us to identify the ActA homologue of the related intracellular motile pathogen, Listeria ivanovii, as a protein with a molecular mass of approximately 150 kDa. VASP also associates with actin filaments recruited by another intracellularly motile bacterial pathogen, Shigella flexneri. Hence, by the simple expedient of expressing surface-bound attractor molecules, bacterial pathogens effectively harness cytoskeletal components to achieve intracellular movement.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.