ArticlePlant molecular biology1995
Existence of two ferredoxin-glutamate synthases in the cyanobacterium Synechocystis sp. PCC 6803. Isolation and insertional inactivation of gltB and gltS genes.
Article in Plant molecular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Synergistic regulation of hydrogen sulfide and nitric oxide on biochemical components, exopolysaccharides, and nitrogen metabolism in nickel stressed rice field cyanobacteria.Journal of plant research · 2024Article
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- Interplay of hydrogen peroxide and nitric oxide: systemic regulation of photosynthetic performance and nitrogen metabolism in cadmium challenged cyanobacteria.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Effect of Time Interval on Arsenic Toxicity to Paddy Field Cyanobacteria as Evident by Nitrogen Metabolism, Biochemical Constituent, and Exopolysaccharide Content.Biological trace element research · 2021Article
- Phycobilisome breakdown effector NblD is required to maintain the cellular amino acid composition during nitrogen starvation.Journal of bacteriology · 2021Article
- Phytohormone up-regulates the biochemical constituent, exopolysaccharide and nitrogen metabolism in paddy-field cyanobacteria exposed to chromium stress.BMC microbiology · 2020Article
- Effects of global transcription factor NtcA on photosynthetic production of ethylene in recombinantBiotechnology for biofuels · 2017Article
- A loop unique to ferredoxin-dependent glutamate synthases is not absolutely essential for ferredoxin-dependent catalytic activity.Photosynthesis research · 2015Article
- Ferredoxin-dependent glutamate synthase: involvement in ammonium assimilation in Haloferax mediterranei.Extremophiles : life under extreme conditions · 2014Article
- Posttranscriptional regulation of glutamine synthetase in the filamentous Cyanobacterium Anabaena sp. PCC 7120: differential expression between vegetative cells and heterocysts.Journal of bacteriology · 2010Article
- Semi-anaerobic growth conditions are favoured by some Escherichia coli strains during heterologous expression of some archaeal proteins.Molecular biology reports · 2006Article
- Glutamate synthase: structural, mechanistic and regulatory properties, and role in the amino acid metabolism.Photosynthesis research · 2005Review
- Ammonium assimilation in cyanobacteria.Photosynthesis research · 2005Review
- Glutamate synthase: an archaeal horizontal gene transfer?Journal of biosciences · 2001Article
- The evolution of glutamate synthase.Molecular biology reports · 2000Article
- Article
- The presence of glutamate dehydrogenase is a selective advantage for the Cyanobacterium synechocystis sp. strain PCC 6803 under nonexponential growth conditions.Journal of bacteriology · 1999Article
- The cyanobacterial thioredoxin gene is required for both photoautotrophic and heterotrophic growth.Plant physiology · 1996Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The first two genes of ferredoxin-dependent glutamate synthase (Fd-GOGAT) from a prokaryotic organism, the cyanobacterium Synechocystis sp. PCC 6803, were cloned in Escherichia coli. Partial sequencing of the cloned genomic DNA, of the 6.3 kb Hind III and 9.3 kb Cla I fragments, confirmed the existence of two different genes coding for glutamate synthases, named gltB and gltS. The gltB gene was completely sequenced and encodes for a polypeptide of 1550 amino acid residues (M(r) 168,964). Comparative analysis of the gltB deduced amino acid sequence against other glutamate synthases shows a higher identity with the alfalfa NADH-GOGAT (55.2%) than with the corresponding Fd-GOGAT from the higher plants maize and spinach (about 43%), the red alga Antithamnion sp. (42%) or with the NADPH-GOGAT of bacterial source, such as Escherichia coli (41%) and Azospirillum brasilense (45%). The detailed analysis of Synechocystis gltB deduced amino acid sequence shows strongly conserved regions that have been assigned to the 3Fe-4S cluster (CX5CHX3C), the FMN-binding domain and the glutamine-amide transferase domain. Insertional inactivation of gltB and gltS genes revealed that both genes code for ferredoxin-dependent glutamate synthases which were nonessential for Synechocystis growth, as shown by the ferredoxin-dependent glutamate synthase activity and western-blot analysis of the mutant strains.
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