ArticleProceedings of the National Academy of Sciences of the United States of America1995
Human general transcription factor TFIIA: characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 72 citations in OpenAlex.
- Natural variations of TFIIAγ gene and LOB1 promoter contribute to citrus canker disease resistance in Atalantia buxifolia.PLoS genetics · 2021Article
- A transcription factor IIA-binding site differentially regulates RNA polymerase II-mediated transcription in a promoter context-dependent manner.The Journal of biological chemistry · 2017Article
- Article
- Taspase1 processing alters TFIIA cofactor properties in the regulation of TFIID.Transcription · 2015Article
- Identification of divergent protein domains by combining HMM-HMM comparisons and co-occurrence detection.PloS one · 2014Article
- Fine time course expression analysis identifies cascades of activation and repression and maps a putative regulator of mammalian sex determination.PLoS genetics · 2013Article
- TFIIB recognition elements control the TFIIA-NC2 axis in transcriptional regulation.Molecular and cellular biology · 2009Article
- Stabilized binding of TBP to the TATA box of herpes simplex virus type 1 early (tk) and late (gC) promoters by TFIIA and ICP4.Journal of virology · 2008Article
- General transcription factor IIA-gamma increases osteoblast-specific osteocalcin gene expression via activating transcription factor 4 and runt-related transcription factor 2.The Journal of biological chemistry · 2008Article
- Interaction of protein inhibitor of activated STAT (PIAS) proteins with the TATA-binding protein, TBP.The Journal of biological chemistry · 2006Article
- Testifying the rice bacterial blight resistance gene xa5 by genetic complementation and further analyzing xa5 (Xa5) in comparison with its homolog TFIIAgamma1.Molecular genetics and genomics : MGG · 2006Article
- Cleavage and proteasome-mediated degradation of the basal transcription factor TFIIA.The EMBO journal · 2004Article
- Differential cellular requirements for activation of herpes simplex virus type 1 early (tk) and late (gC) promoters by ICP4.Journal of virology · 2004Article
- Structural and functional interactions of transcription factor (TF) IIA with TFIIE and TFIIF in transcription initiation by RNA polymerase II.The Journal of biological chemistry · 2001Article
- Inhibition of androgen receptor-mediated transcription by amino-terminal enhancer of split.Molecular and cellular biology · 2001Article
- Binding of TATA binding protein to a naturally positioned nucleosome is facilitated by histone acetylation.Molecular and cellular biology · 2001Article
- BRCA1 interaction with RNA polymerase II reveals a role for hRPB2 and hRPB10alpha in activated transcription.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Analysis of TFIIA function In vivo: evidence for a role in TATA-binding protein recruitment and gene-specific activation.Molecular and cellular biology · 1999Article
- Phosphorylation of TFIIA stimulates TATA binding protein-TATA interaction and contributes to maximal transcription and viability in yeast.Molecular and cellular biology · 1999Article
- The general transcription factors IIA, IIB, IIF, and IIE are required for RNA polymerase II transcription from the human U1 small nuclear RNA promoter.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The human general transcription factor TFIIA is one of several factors involved in specific transcription by RNA polymerase II, possibly by regulating the activity of the TATA-binding subunit (TBP) of TFIID. TFIIA purified from HeLa extracts consists of 35-, 19-, and 12-kDa subunits. Here we describe the isolation of a cDNA clone (hTFIIA gamma) encoding the 12-kDa subunit. Using expression constructs derived from hTFIIA gamma and TFIIA alpha/beta (which encodes a 55-kDa precursor to the alpha and beta subunits of natural TFIIA), we have constructed a synthetic TFIIA with a polypeptide composition similar to that of natural TFIIA. The recombinant complex supports the formation of a DNA-TBP-TFIIA complex and mediates both basal and Gal4-VP16-activated transcription by RNA polymerase II in TFIIA-depleted nuclear extracts. In contrast, TFIIA has no effect on tRNA and 5S RNA transcription by RNA polymerase III in this system. We also present evidence that both the p55 and p12 recombinant subunits interact with TBP and that the basic region of TBP is critical for the TFIIA-dependent function of TBP in nuclear extracts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.