ArticleThe Journal of experimental medicine1995
The intracellular signal transduction mechanism of interleukin 5 in eosinophils: the involvement of lyn tyrosine kinase and the Ras-Raf-1-MEK-microtubule-associated protein kinase pathway.
Article in The Journal of experimental medicine, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 162 citations in OpenAlex.
- TLR-7 Stress Signaling in Differentiating and Mature Eosinophils Is Mediated by the Prolyl Isomerase Pin1.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- mTOR complexes differentially orchestrates eosinophil development in allergy.Scientific reports · 2018Article
- Benralizumab: From the Basic Mechanism of Action to the Potential Use in the Biological Therapy of Severe Eosinophilic Asthma.BioMed research international · 2018Review
- Benralizumab in the treatment of severe asthma: design, development and potential place in therapy.Drug design, development and therapy · 2018Review
- Eosinophil differentiation in the bone marrow is promoted by protein tyrosine phosphatase SHP2.Cell death & disease · 2016Article
- Evaluation of peroxisome proliferator-activated receptor agonists on interleukin-5-induced eosinophil differentiation.Immunology · 2014Article
- Regulation of spontaneous eosinophil apoptosis-a neglected area of importance.Journal of cell death · 2014Review
- Application of multiplexed kinase inhibitor beads to study kinome adaptations in drug-resistant leukemia.PloS one · 2013Article
- Lyn is involved in CD24-induced ERK1/2 activation in colorectal cancer.Molecular cancer · 2012Article
- Identification of pro-inflammatory cytokines associated with muscle invasive bladder cancer; the roles of IL-5, IL-20, and IL-28A.PloS one · 2012Article
- Balanced interactions between Lyn, the p85alpha regulatory subunit of class I(A) phosphatidylinositol-3-kinase, and SHIP are essential for mast cell growth and maturation.Molecular and cellular biology · 2011Article
- Mitogen-activated protein kinase signalling and ERK1/2 bistability in asthma.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2011Review
- Chemoattractant-induced signaling via the Ras-ERK and PI3K-Akt networks, along with leukotriene C4 release, is dependent on the tyrosine kinase Lyn in IL-5- and IL-3-primed human blood eosinophils.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Interleukin-5 and IL-5 receptor in health and diseases.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2011Review
- Fc receptor gamma-chain, a constitutive component of the IL-3 receptor, is required for IL-3-induced IL-4 production in basophils.Nature immunology · 2009Article
- trans-Resveratrol, an extract of red wine, inhibits human eosinophil activation and degranulation.British journal of pharmacology · 2008Article
- In vitro effects of budesonide on eosinophil-basophil lineage commitment.The open respiratory medicine journal · 2008Article
- Spred-1 negatively regulates allergen-induced airway eosinophilia and hyperresponsiveness.The Journal of experimental medicine · 2005Article
- Interleukin-6-induced proliferation of human myeloma cells associated with CD45 molecules.International journal of hematology · 2003Review
- A parallel signal-transduction pathway for eotaxin- and interleukin-5-induced eosinophil shape change.Immunology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Interleukin 5 (IL-5) regulates the growth and function of eosinophils. The objective of this study was to investigate the intracellular signal transduction mechanism of IL-5 in eosinophils. Purified eosinophils were stimulated with IL-5, and the involvement of various kinases was investigated by immunoblotting, immune complex kinase assay, and in situ denatured/renatured kinase assay. We found that IL-5 induced tyrosine phosphorylation and activation of a number of kinases. Two species of lyn kinases (53 and 56 kD) were present in eosinophils. Both forms were Tyr-phosphorylated and activated rapidly within 1 min. Further, lyn kinase was physically associated with the IL-5 beta receptor in eosinophils. Ras was studied by immunoprecipitation followed by thin-layer chromatography. Ras bound higher quantities of [alpha-32P]guanosine 5'triphosphate upon stimulation with IL-5. Raf-1 kinase showed increased Tyr phosphorylation on immunoblotting and increased activity in the immune complex kinase assay. Two species of MEK (MAP or Erk kinase) (41 and 45 kD) were identified in eosinophils, which underwent autophosphorylation upon stimulation. Microtubule-associated protein (MAP) kinase (p44) was Tyr-phosphorylated on immunoblotting and had increased activity in the immune-complex kinase assay. MAP kinases were also studied after metabolic radiolabeling of the cells with [32P]orthophosphates. IL-5 stimulated phosphorylation of MAP kinases in situ. Thus, we have delineated major components of an important signaling pathway in eosinophils. We believe that one of the signals generated by IL-5 receptor activation is propagated through the lyn-Ras-Raf-1-MEK-MAP kinase pathway.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.