ArticleThe Journal of experimental medicine1995
Intracellular targeting of antigens internalized by membrane immunoglobulin in B lymphocytes.
Article in The Journal of experimental medicine, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 91 citations in OpenAlex.
- Deep mutational scanning reveals transmembrane features governing surface expression of the B cell antigen receptor.Frontiers in immunology · 2024Article
- The immunobiology of ubiquitin-dependent B cell receptor functions.Molecular immunology · 2018Review
- Efficient generation of antigen-specific CTLs by the BAFF-activated human B Lymphocytes as APCs: a novel approach for immunotherapy.Oncotarget · 2016Article
- Antigen-B Cell Receptor Complexes Associate with Intracellular major histocompatibility complex (MHC) Class II Molecules.The Journal of biological chemistry · 2015Article
- The Syk-binding ubiquitin ligase c-Cbl mediates signaling-dependent B cell receptor ubiquitination and B cell receptor-mediated antigen processing and presentation.The Journal of biological chemistry · 2012Article
- Identification of CD4+ T cell epitopes in C. burnetii antigens targeted by antibody responses.PloS one · 2011Article
- Independent trafficking of Ig-alpha/Ig-beta and mu-heavy chain is facilitated by dissociation of the B cell antigen receptor complex.Journal of immunology (Baltimore, Md. : 1950) · 2005Article
- Models of signal transduction through the B-cell antigen receptor.Immunology · 2003Review
- Role of the BCR complex in B cell development, activation, and leukemic transformation.Immunologic research · 2003Review
- Antigen binding to GM1 ganglioside results in delayed presentation: minimal effects of GM1 on presentation of antigens internalized via other pathways.Immunology · 2002Article
- Aberrant B cell receptor signaling from B29 (Igbeta, CD79b) gene mutations of chronic lymphocytic leukemia B cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Modulation of the major histocompatibility complex class II-associated peptide repertoire by human histocompatibility leukocyte antigen (HLA)-DO.The Journal of experimental medicine · 2000Article
- A role for lipid rafts in B cell antigen receptor signaling and antigen targeting.The Journal of experimental medicine · 1999Article
- Purification of intracellular compartments involved in antigen processing: a new method based on magnetic sorting.The Biochemical journal · 1999Article
- The monomeric guanosine triphosphatase rab4 controls an essential step on the pathway of receptor-mediated antigen processing in B cells.The Journal of experimental medicine · 1998Article
- Syk tyrosine kinase and B cell antigen receptor (BCR) immunoglobulin-alpha subunit determine BCR-mediated major histocompatibility complex class II-restricted antigen presentation.The Journal of experimental medicine · 1998Article
- Distinct intracellular compartments involved in invariant chain degradation and antigenic peptide loading of major histocompatibility complex (MHC) class II molecules.The Journal of cell biology · 1997Article
- CD40-activated human B cells: an alternative source of highly efficient antigen presenting cells to generate autologous antigen-specific T cells for adoptive immunotherapy.The Journal of clinical investigation · 1997Article
- Delivery of B cell receptor-internalized antigen to endosomes and class II vesicles.The Journal of experimental medicine · 1997Article
- Antigen endocytosis and presentation mediated by human membrane IgG1 in the absence of the Ig(alpha)/Ig(beta) dimer.The EMBO journal · 1997Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
An important function of membrane immunoglobulin (mIg), the B cell antigen receptor, is to endocytose limiting quantities of antigen for efficient presentation to class II-restricted T cells. We have used a panel of mIg mutants to analyze the mechanism of mIg-mediated antigen presentation, and specifically to explore the ability of mIg to target internalized antigen to intracellular processing compartments. Transfected mIgs carrying substitutions for the transmembrane Tyr587 residue fail to efficiently present specifically bound antigen. However, these mutants internalize antigen normally, and their defect cannot be attributed to a lack of mIg-associated Ig alpha/Ig beta molecules. A novel functional assay for detecting antigenic peptides in subcellular fractions shows that wild-type mIg transfectants generate class II-peptide complexes intracellularly, whereas only free antigenic peptides are detectable in the mutant mIg transfectants. Furthermore, an antigen competition assay reveals that antigen internalized by the mutant mIgs fails to enter the intracellular processing compartment accessed by wild-type mIg. Therefore, mIg specifically targets bound and endocytosed antigen to the intracellular compartment where processed peptides associate with class II molecules, and the transmembrane Tyr587 residue plays an obligatory role in this process. Targeting of internalized antigen may be mediated by receptor-associated chaperones, and may be a general mechanism for optimizing the presentation of specifically bound and endocytosed antigens in b lymphocytes and other antigen-presenting cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.