ArticleThe Journal of cell biology1995
Two distinct functions of the carboxyl-terminal tail domain of NF-M upon neurofilament assembly: cross-bridge formation and longitudinal elongation of filaments.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 114 citations in OpenAlex.
- Neurofilaments in health and Charcot-Marie-Tooth disease.Frontiers in cell and developmental biology · 2023Review
- A review and analysis of the clinical literature on Charcot-Marie-Tooth disease caused by mutations in neurofilament protein L.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Chemotherapy-Induced Peripheral Neuropathy and Changes in Cytoskeleton.International journal of molecular sciences · 2019Review
- Axonal neurofilaments exhibit frequent and complex folding behaviors.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Neurofilaments and Neurofilament Proteins in Health and Disease.Cold Spring Harbor perspectives in biology · 2017Review
- Targeting caspase-6 and caspase-8 to promote neuronal survival following ischemic stroke.Cell death & disease · 2015Article
- Article
- Human intraretinal myelination: axon diameters and axon/myelin thickness ratios.Indian journal of ophthalmology · 2013Article
- Article
- Assembly properties of lamprey neurofilament subunits and their expression after spinal cord transection.The Journal of comparative neurology · 2011Article
- Phosphatidylinositol phosphates directly bind to neurofilament light chain (NF-L) for the regulation of NF-L self assembly.Experimental & molecular medicine · 2011Article
- The polymer brush model of neurofilament projections: effect of protein composition.Biophysical journal · 2010Article
- Phosphorylation of highly conserved neurofilament medium KSP repeats is not required for myelin-dependent radial axonal growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Electron tomographic analysis of cytoskeletal cross-bridges in the paranodal region of the node of Ranvier in peripheral nerves.Journal of structural biology · 2008Article
- Neurotrophins and peripheral neuropathies.Brain pathology (Zurich, Switzerland) · 2006Review
- Spatio-temporal changes in neurofilament proteins immunoreactivity following kainate-induced cerebellar lesion in rats.Cellular and molecular neurobiology · 2004Article
- The neurofilament middle molecular mass subunit carboxyl-terminal tail domains is essential for the radial growth and cytoskeletal architecture of axons but not for regulating neurofilament transport rate.The Journal of cell biology · 2003Article
- NF-M is an essential target for the myelin-directed "outside-in" signaling cascade that mediates radial axonal growth.The Journal of cell biology · 2003Article
- A role for intermediate filaments in determining and maintaining the shape of nerve cells.Molecular biology of the cell · 2003Article
- Neurofilament transport in vivo minimally requires hetero-oligomer formation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurofilaments are the major cytoskeletal elements in the axon that take highly ordered structures composed of parallel arrays of 10-nm filaments linked to each other with frequent cross-bridges, and they are believed to maintain a highly polarized neuronal cell shape. Here we report the function of rat NF-M in this characteristic neurofilament assembly. Transfection experiments were done in an insect Sf9 cell line lacking endogenous intermediate filaments. NF-L and NF-M coassemble to form bundles of 10-nm filaments packed in a parallel manner with frequent cross-bridges resembling the neurofilament domains in the axon when expressed together in Sf9 cells. Considering the fact that the expression of either NF-L or NF-M alone in these cells results in neither formation of any ordered network of 10-nm filaments nor cross-bridge structures, NF-M plays a crucial role in this parallel filament assembly. In the case of NF-H the carboxyl-tail domain has been shown to constitute the cross-bridge structures. The similarity in molecular architecture between NF-M and NF-H suggests that the carboxyl-terminal tail domain of NF-M also constitutes cross-bridges. To examine this and to further investigate the function of the carboxyl-terminal tail domain of NF-M, we made various deletion mutants that lacked part of their tail domains, and we expressed these with NF-L. From this deletion mutant analysis, we conclude that the carboxyl-terminal tail domain of NF-M has two distinct functions. First, it is the structural component of cross-bridges, and these cross-bridges serve to control the spacing between core filaments. Second, the portion of the carboxyl-terminal tail domain of NF-M that is directly involved in cross-bridge formation affects the core filament assembly by helping them to elongate longitudinally so that they become straight.
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