ArticlePlant physiology1995
The effects of mastoparan on the carrot cell plasma membrane polyphosphoinositide phospholipase C.
Article in Plant physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 59 citations in OpenAlex.
- Mastoparans: A Group of Multifunctional α-Helical Peptides With Promising Therapeutic Properties.Frontiers in molecular biosciences · 2022Review
- Membrane-associated phosphoinositides-specific phospholipase C forms from Catharanthus roseus transformed roots.Molecular biotechnology · 2007Article
- Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C.Plant physiology · 2002Review
- Up-regulation of phosphoinositide metabolism in tobacco cells constitutively expressing the human type I inositol polyphosphate 5-phosphatase.Plant physiology · 2002Article
- Induction of lipid metabolic enzymes during the endoplasmic reticulum stress response in plants.Plant physiology · 2001Article
- Rice dwarf mutant d1, which is defective in the alpha subunit of the heterotrimeric G protein, affects gibberellin signal transduction.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Article
- The relationship between photosynthesis and a mastoparan-induced hypersensitive response in isolated mesophyll cellsPlant physiology · 1999Article
- Phosphoglycerylethanolamine posttranslational modification of plant eukaryotic elongation factor 1alpha.Plant physiology · 1998Article
- Heterotrimeric G proteins are implicated in gibberellin induction of a-amylase gene expression in wild oat aleuroneThe Plant cell · 1998Article
- Metabolic evidence for PtdIns(4,5)P2-directed phospholipase C in permeabilized plant protoplasts.The Biochemical journal · 1997Article
- Novel Phosphoinositides in Barley Aleurone Cells (Additional Evidence for the Presence of Phosphatidyl-scyllo-Inositol).Plant physiology · 1997Article
- Growth of Pollen Tubes of Papaver rhoeas Is Regulated by a Slow-Moving Calcium Wave Propagated by Inositol 1,4,5-Trisphosphate.The Plant cell · 1996Article
- Inositol phosphates in the duckweed Spirodela polyrhiza L.The Biochemical journal · 1996Article
- G Protein Activation Stimulates Phospholipase D Signaling in Plants.The Plant cell · 1995Article
- Evidence for substrate-cycling of 3-, 3,4-, 4-, and 4,5-phosphorylated phosphatidylinositols in plants.The Biochemical journal · 1995Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When [3H]inositol-labeled carrot (Daucus carota L.) cells were treated with 10 or 25 microM wasp venom peptide mastoparan or the active analog Mas-7 there was a rapid loss of more than 70% of [3H]phosphatidylinositol-4-monophosphate (PIP) and [3H]phosphatidylinositol-4,5-bisphosphate (PIP2) and a 3- and 4-fold increase in [3H]inositol-1,4-P2 and [3H]inositol-1,4,5-P3, respectively. The identity of [3H]inositol-1,4,5-P3 was confirmed by phosphorylation with inositol-1,4,5-P3 3-kinase and co-migration with inositol-1,3,4,5-P4. The changes in phosphoinositides were evident within 1 min. The loss of [3H]PIP was evident only when cells were treated with the higher concentrations (10 and 25 microM) of mastoparan or Mas-7. At 1 microM Mas-7, [3H]PIP increased. The inactive mastoparan analog Mas-17 had little or no effect on [3H]PIP or [3H]PIP2 hydrolysis in vivo. Neomycin (100 microM) inhibited the uptake of Mas-7 and thereby inhibited the Mas-7-stimulated hydrolysis of [3H]PIP and [3H]PIP2. Plasma membranes isolated from mastoparan-treated cells had increased PIP-phospholipase C (PLC) activity. However, when Mas-7 was added to isolated plasma membranes from control cells, it had no effect on PIP-PLC activity at low concentrations and inhibited PIP-PLC at concentrations greater than 10 microM. In addition, guanosine-5'-O-(3-thiotriphosphate) had no effect on the PIP-PLC activity when added to plasma membranes isolated from either the Mas-7-treated or control cells. The fact that Mas-7 did not stimulate PIP-PLC activity in vitro indicated that the Mas-7-induced increase in PIP-PLC in vivo required a factor that was lost from the membrane during isolation.
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