ArticleMolecular & general genetics : MGG1995
The cycHJKL genes of Rhizobium meliloti involved in cytochrome c biogenesis are required for "respiratory" nitrate reduction ex planta and for nitrogen fixation during symbiosis.
Article in Molecular & general genetics : MGG, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 58 citations in OpenAlex.
- CcmI subunit of CcmFHI heme ligation complex functions as an apocytochrome c chaperone during c-type cytochrome maturation.The Journal of biological chemistry · 2011Article
- How rhizobial symbionts invade plants: the Sinorhizobium-Medicago model.Nature reviews. Microbiology · 2007Review
- Identification of novel Sinorhizobium meliloti mutants compromised for oxidative stress protection and symbiosis.Journal of bacteriology · 2007Article
- The roles of different regions of the CycH protein in c-type cytochrome biogenesis in Sinorhizobium meliloti.Molecular genetics and genomics : MGG · 2004Article
- Brucella abortus HtrA functions as an authentic stress response protease but is not required for wild-type virulence in BALB/c mice.Infection and immunity · 2001Article
- Identification of Legionella pneumophila genes important for infection of amoebas by signature-tagged mutagenesis.Infection and immunity · 2001Article
- A high-density physical map of Sinorhizobium meliloti 1021 chromosome derived from bacterial artificial chromosome library.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Novel rkp gene clusters of Sinorhizobium meliloti involved in capsular polysaccharide production and invasion of the symbiotic nodule: the rkpK gene encodes a UDP-glucose dehydrogenase.Journal of bacteriology · 1998Article
- Transcriptional control and essential roles of the Escherichia coli ccm gene products in formate-dependent nitrite reduction and cytochrome c synthesis.The Biochemical journal · 1998Article
- The active-site cysteines of the periplasmic thioredoxin-like protein CcmG of Escherichia coli are important but not essential for cytochrome c maturation in vivo.Journal of bacteriology · 1998Article
- Cell biology and molecular basis of denitrification.Microbiology and molecular biology reviews : MMBR · 1997Review
- Biogenesis of respiratory cytochromes in bacteria.Microbiology and molecular biology reviews : MMBR · 1997Review
- The role of the genes nrf EFG and ccmFH in cytochrome c biosynthesis in Escherichia coli.Molecular & general genetics : MGG · 1996Article
- Rhodobacter capsulatus CycH: a bipartite gene product with pleiotropic effects on the biogenesis of structurally different c-type cytochromes.Journal of bacteriology · 1996Article
- Heme synthesis in the rhizobium-legume symbiosis: a palette for bacterial and eukaryotic pigments.Journal of bacteriology · 1996Review
- Genetic analysis of Rhizobium meliloti bacA-phoA fusion results in identification of degP: two loci required for symbiosis are closely linked to degP.Journal of bacteriology · 1996Article
- Characterization of the cycHJKL genes involved in cytochrome c biogenesis and symbiotic nitrogen fixation in Rhizobium leguminosarum.Journal of bacteriology · 1995Article
- Escherichia coli genes required for cytochrome c maturation.Journal of bacteriology · 1995Article
- The cycHJKL gene cluster plays an essential role in the biogenesis of c-type cytochromes in Bradyrhizobium japonicum.Molecular & general genetics : MGG · 1995Article
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5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the genetic and biochemical analysis of Rhizobium meliloti mutants defective in symbiotic nitrogen fixation (Fix-) and "respiratory" nitrate reduction (Rnr-). The mutations were mapped close to the ade-1 and cys-46 chromosomal markers and the mutated locus proved to be identical to the previously described fix-14 locus. By directed Tn5 mutagenesis, a 4.5 kb segment of the chromosome was delimited in which all mutations resulted in Rnr- and Fix- phenotypes. Nucleotide sequence analysis of this region revealed the presence of four open reading frames coding for integral membrane and membrane-anchored proteins. Biochemical analysis of the mutants showed that the four proteins were necessary for the biogenesis of all cellular c-type cytochromes. In agreement with the nomenclature proposed for rhizobial genes involved in the formation of c-type cytochromes, the four genes were designated cycH, cycJ, cycK, and cycL, respectively. The predicted protein product of cycH exhibited a high degree of similarity to the Bradyrhizobium japonicum counterpart, while CycK and CycL shared more than 50% amino acid sequence identity with the Rhodobacter capsulatus Cc11 and Cc12 proteins, respectively. cycJ encodes a novel membrane anchored protein of 150 amino acids. We suggest that this gene cluster codes for (parts of) a multisubunit cytochrome c haem lyase. Moreover, our results indicate that in R. meliloti c-type cytochromes are required for respiratory nitrate reduction ex planta, as well as for symbiotic nitrogen fixation in root nodules.
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