ArticleGenetics1995
An inverse PCR screen for the detection of P element insertions in cloned genomic intervals in Drosophila melanogaster.
Article in Genetics, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- A critical component of meiotic drive in Neurospora is located near a chromosome rearrangement.Genetics · 2014Article
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- A forward genetic screen in Drosophila implicates insulin signaling in age-related locomotor impairment.Experimental gerontology · 2009Article
- Genetic dissociation of ethanol sensitivity and memory formation in Drosophila melanogaster.Genetics · 2008Article
- DmOAZ, the unique Drosophila melanogaster OAZ homologue is involved in posterior spiracle development.Development genes and evolution · 2007Article
- Drosophila Eph receptor guides specific axon branches of mushroom body neurons.Development (Cambridge, England) · 2006Article
- DNase II deficiency impairs innate immune function in Drosophila.Cellular immunology · 2006Article
- Flexibility in a gene network affecting a simple behavior in Drosophila melanogaster.Genetics · 2005Article
- Creation of EcR isoform-specific mutations in Drosophila melanogaster via local P element transposition, imprecise P element excision, and male recombination.Molecular genetics and genomics : MGG · 2004Article
- MKP-3 has essential roles as a negative regulator of the Ras/mitogen-activated protein kinase pathway during Drosophila development.Molecular and cellular biology · 2004Article
- The clot gene of Drosophila melanogaster encodes a conserved member of the thioredoxin-like protein superfamily.Molecular genetics and genomics : MGG · 2003Article
- The roX genes encode redundant male-specific lethal transcripts required for targeting of the MSL complex.The EMBO journal · 2002Article
- Screening insertion libraries for mutations in many genes simultaneously using DNA microarrays.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Requirement of RBP9, a Drosophila Hu homolog, for regulation of cystocyte differentiation and oocyte determination during oogenesis.Molecular and cellular biology · 1999Article
- The role of RBF in the introduction of G1 regulation during Drosophila embryogenesis.The EMBO journal · 1999Article
- Article
- Kinesin light chains are essential for axonal transport in Drosophila.The Journal of cell biology · 1998Article
- Biological characterization of Drosophila Rapgap1, a GTPase activating protein for Rap1.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Genetic analysis of myoblast fusion: blown fuse is required for progression beyond the prefusion complex.The Journal of cell biology · 1997Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a screening approach that utilizes an inverse polymerase chain reaction (PCR) to detect P element insertions in or near previously cloned genes in Drosophila melanogaster. We used this approach in a large scale genetic screen in which P elements were mobilized from sites on the X chromosome to new autosomal locations. Mutagenized flies were combined in pools, and our screening approach was used to generate probes corresponding to the sequences flanking each site of insertion. These probes then were used for hybridization to cloned genomic intervals, allowing individuals carrying insertions in them to be detected. We used the same approach to perform repeated rounds of sib-selection to generate stable insertion lines. We screened 16,100 insert bearing individuals and recovered 11 insertions in five intervals containing genes encoding members of the kinesin superfamily in Drosophila melanogaster. In addition, we recovered an insertion in the region including the Larval Serum Protein-2 gene. Examination by Southern hybridization confirms that the lines we recovered represent genuine insertions in the corresponding genomic intervals. Our data indicates that this approach will be very efficient both for P element mutagenesis of new genomic regions and for detection and recovery of "local" P element transposition events. In addition, our data constitutes a survey of preferred P element insertion sites in the Drosophila genome and suggests that insertion sites that are mutable at a rate of approximately 10(-4) are distributed every 40-50 kb.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.