ArticleBiophysical journal1995
Metal-ligand complexes as a new class of long-lived fluorophores for protein hydrodynamics.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 196 citations in OpenAlex.
- DFT Insights on Ligand Photodissociation Pathways in Ruthenium-Terpyridine Complexes:Inorganic chemistry · 2026Article
- Ruthenium Complexes in Protein Science: Heading a New Generation of Therapeutic and Analytical Tools.Angewandte Chemie (International ed. in English) · 2026Review
- Charge engineering controls cooperative assembly and loading in protein host-guest complexes.Journal of materials chemistry. B · 2025Article
- Tracking of fluorescent antibiotic conjugate in planta utilizing fluorescence lifetime imaging.Planta · 2021Article
- Article
- Targeting the mitochondrial VDAC in hepatocellular carcinoma using a polyclonal antibody-conjugated to a nitrosyl ruthenium complex.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Article
- Applications of Ruthenium Complexes Covalently Linked to Nucleic Acid Derivatives.Molecules (Basel, Switzerland) · 2018Review
- Hydrodynamic Radii of Ranibizumab, Aflibercept and Bevacizumab Measured by Time-Resolved Phosphorescence Anisotropy.Pharmaceutical research · 2016Article
- Ru(II)-diimine functionalized metalloproteins: From electron transfer studies to light-driven biocatalysis.Biochimica et biophysica acta · 2016Review
- Fluorescence polarization assays in high-throughput screening and drug discovery: a review.Methods and applications in fluorescence · 2016Article
- Synthesis, labeling and bioanalytical applications of a tris(2,2'-bipyridyl)ruthenium(II)-based electrochemiluminescence probe.Nature protocols · 2014Article
- Coomassie blue as a near-infrared fluorescent stain: a systematic comparison with Sypro Ruby for in-gel protein detection.Molecular & cellular proteomics : MCP · 2013Article
- Photophysical studies of bioconjugated ruthenium metal-ligand complexes incorporated in phospholipid membrane bilayers.Inorganic chemistry · 2013Article
- Developing a Fluorescence-based Approach to Screening for Macromolecule Crystallization Conditions.Crystal growth & design · 2011Article
- Dynamics of bacteriophage R17 probed with a long-lifetime Ru(II) metal-ligand complex.Journal of fluorescence · 2010Article
- Tuning photophysical properties with ancillary ligands in Ru(II) mono-diimine complexes.Journal of organometallic chemistry · 2009Article
- A high throughput fluorescence polarization assay for inhibitors of the GoLoco motif/G-alpha interaction.Combinatorial chemistry & high throughput screening · 2008Article
- PCR-free quantitative detection of genetically modified organism from raw materials. An electrochemiluminescence-based bio bar code method.Analytical chemistry · 2008Article
- Interactions between CLIP-170, tubulin, and microtubules: implications for the mechanism of Clip-170 plus-end tracking behavior.Molecular biology of the cell · 2005Article
- Effects of metallic silver particles on the emission properties of [Ru(bpy)(3)].Chemical physics letters · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We describe the use of asymmetric Ru-ligand complexes as a new class of luminescent probes that can be used to measure rotational motions of proteins. These complexes are known to display luminescent lifetimes ranging from 10 to 4000 ns. In this report, we show that the asymmetric complex Ru(bpy)2(dcbpy) (PF6)2 displays a high anisotropy value when excited in the long wavelength absorption band. For covalent linkage to proteins, we synthesized the N-hydroxy succinimide ester of this metal-ligand complex. To illustrate the usefulness of these probes, we describe the intensity and anisotropy decays of [Ru(bpy)2(dcbpy)] when covalently linked to human serum albumin, concanavalin A (ConA), human immunoglobulin G (IgG), and Ferritin, and measured in solutions of increased viscosity. These data demonstrate that the probes can be used to measure rotational motions on the 10 ns to 1.5 microseconds timescale, which so far has been inaccessible using luminescence methods. The present probe [Ru(bpy)2(dcbpy)] can be regarded as the first of a class of metal-ligand complexes, each with different chemical reactivity and spectral properties, for studies of macromolecular dynamics.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.