ArticleProceedings of the National Academy of Sciences of the United States of America1995
A well-differentiated B-cell line is permissive for somatic mutation of a transfected immunoglobulin heavy-chain gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- The rate of mutation of a single gene.Nucleic acids research · 2010Article
- Somatic hypermutation of the AID transgene in B and non-B cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- In vivo and in vitro studies of immunoglobulin gene somatic hypermutation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2001Review
- The Ig mutator is dependent on the presence, position, and orientation of the large intron enhancer.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Sequence dependent hypermutation of the immunoglobulin heavy chain in cultured B cells.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- The promotion of V region hypermutation.The Journal of experimental medicine · 1997Article
- Differential V region mutation of two transfected Ig genes and their interaction in cultured B cell lines.The EMBO journal · 1996Article
- An immunoglobulin mutator that targets G.C base pairs.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Immunoglobulin variable region hypermutation in hybrids derived from a pre-B- and a myeloma cell line.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
pSV2neo plasmids containing an IgM heavy-chain gene with nonsense mutations in either the variable (V) or the constant (C) region were transfected into four differentiated mouse plasma cell lines: S107 and the NSO fusion partner (myeloma cell lines) and 2C3 and 36.65 (hybridoma cell lines). The frequencies of reversion of the nonsense mutations in multiple independent transfectants were determined with the spot ELISA and rates of reversion were calculated by fluctuation analysis. Mutations in both V and C regions were confirmed by sequence analyses. In the S107 cell line, spontaneous point mutations occurred in the V region at a rate of approximately 5 x 10(-5)/bp per cell generation, > 400-fold higher than the rate of V-region mutation in the NSO cell line and considerably higher than the rates in 2C3 and 36.65 hybridoma cell lines. These studies suggest that S107 is a relatively permissive cell line in which V-region mutations can occur constitutively, even though it represents a late stage of B-cell differentiation. Further, the results show that the construct used contains sufficient information in its flanking and coding sequences to allow a relatively high rate of V-region mutation, at least in the S107 cell line.
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