ArticleProceedings of the National Academy of Sciences of the United States of America1995
Use of Saccharomyces cerevisiae for patch-clamp analysis of heterologous membrane proteins: characterization of Kat1, an inward-rectifying K+ channel from Arabidopsis thaliana, and comparison with endogeneous yeast channels and carriers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 96 citations in OpenAlex.
- Rice cultivars with differing salt tolerance contain similar cation channels in their root cells.Journal of experimental botany · 2012Article
- Competition between uptake of ammonium and potassium in barley and Arabidopsis roots: molecular mechanisms and physiological consequences.Journal of experimental botany · 2010Article
- Heterelogous expression of plant genes.International journal of plant genomics · 2009Article
- Properties of shaker-type potassium channels in higher plants.The Journal of membrane biology · 2006Review
- A procedure for localisation and electrophysiological characterisation of ion channels heterologously expressed in a plant context.Plant methods · 2005Article
- Functions of AKT1 and AKT2 potassium channels determined by studies of single and double mutants of Arabidopsis.Plant physiology · 2001Article
- Expression and stress-dependent induction of potassium channel transcripts in the common ice plant.Plant physiology · 2001Article
- Rundown of the hyperpolarization-activated KAT1 channel involves slowing of the opening transitions regulated by phosphorylation.Biophysical journal · 1999Article
- Divalent cation block of inward currents and low-affinity K+ uptake in Saccharomyces cerevisiae.Journal of bacteriology · 1999Article
- Molecular cloning and expression characterization of a rice K+ channel beta subunit.Plant molecular biology · 1998Article
- Guard cells possess a calcium-dependent protein kinase that phosphorylates the KAT1 potassium channel.Plant physiology · 1998Article
- AtKuP1: a dual-affinity K+ transporter from Arabidopsis.The Plant cell · 1998Article
- The spTRK gene encodes a potassium-specific transport protein TKHp in Schizosaccharomyces pombe.The Journal of membrane biology · 1997Article
- Random mutagenesis reveals a region important for gating of the yeast K+ channel Ykc1.The EMBO journal · 1997Article
- Electrorotation of single yeast cells at frequencies between 100 Hz and 1.6 GHz.Biophysical journal · 1997Article
- Plant K+ channel alpha-subunits assemble indiscriminately.Biophysical journal · 1997Article
- Voltage-dependent gating characteristics of the K+ channel KAT1 depend on the N and C termini.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Potassium transport in Schizosaccharomyces pombe.Folia microbiologica · 1997Article
- Amino acid substitutions in membrane-spanning domains of Hol1, a member of the major facilitator superfamily of transporters, confer nonselective cation uptake in Saccharomyces cerevisiae.Journal of bacteriology · 1996Article
- Changes in voltage activation, Cs+ sensitivity, and ion permeability in H5 mutants of the plant K+ channel KAT1.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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4 authors at 1 institution in 1 country.
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Abstract
Transport-deficient strains of the yeast Saccharomyces cerevisiae have recently proven useful for cloning, by functional complementation, of cDNAs encoding heterologous membrane transporters: specifically, H(+)-amino acid symporters and K+ channels from the higher plant Arabidopsis thaliana. The present study uses whole-cell patch-clamp experiments to show that yeast strains which grow poorly on submillimolar K+ due to the deletion of two K(+)-transporter genes (TRK1 and TRK2) are in fact missing a prominent K+ inward current present in wild-type cells. Rescue of such strains for growth on low K+ by transformation with a gene (KAT1) encoding an inward-rectifying K+ channel from Arabidopsis is accompanied by the appearance of an inward current whose characteristics are in qualitative agreement with previous studies in the Xenopus oocyte system, but differ in quantitative details. The ability to make such measurements directly on Saccharomyces should facilitate structure-function studies of any electrogenic or electrophoretic ion transporters which can be expressed in the plasma membrane (or tonoplast) of that organism.
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