ArticleProceedings of the National Academy of Sciences of the United States of America1995
Human autoantibody recognition of DNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 122 citations in OpenAlex.
- Prothrombotic antibodies targeting the spike protein's receptor-binding domain in severe COVID-19.Blood · 2025Article
- IRAK4 is an immunological checkpoint in neuropsychiatric systemic lupus erythematosus.Scientific reports · 2024Article
- Review
- Antigen pressure from two founder viruses induces multiple insertions at a single antibody position to generate broadly neutralizing HIV antibodies.PLoS pathogens · 2023Article
- HOT or not: examining the basis of high-occupancy target regions.Nucleic acids research · 2019Article
- Loss of an IgG plasma cell checkpoint in patients with lupus.The Journal of allergy and clinical immunology · 2019Article
- Single-Molecule Interactions of a Monoclonal Anti-DNA Antibody with DNA.BioNanoScience · 2017Article
- Overlapping hotspots in CDRs are critical sites for V region diversification.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Immunoglobulin heavy variable (IGHV) genes and alleles: new entities, new names and implications for research and prognostication in chronic lymphocytic leukaemia.Immunogenetics · 2015Article
- Stabilised DNA secondary structures with increasing transcription localise hypermutable bases for somatic hypermutation in IGHV3-23.Immunogenetics · 2012Article
- Over 30% of patients with splenic marginal zone lymphoma express the same immunoglobulin heavy variable gene: ontogenetic implications.Leukemia · 2012Article
- Immunoglobulin gene repertoire in chronic lymphocytic leukemia: insight into antigen selection and microenvironmental interactions.Mediterranean journal of hematology and infectious diseases · 2012Article
- Altered spectrum of somatic hypermutation in common variable immunodeficiency disease characteristic of defective repair of mutations.Immunogenetics · 2011Article
- Specific cross-reaction of anti-dsDNA antibody with platelet integrin GPIIIa49-66.Autoimmunity · 2010Article
- Development of self-reactive germinal center B cells and plasma cells in autoimmune Fc gammaRIIB-deficient mice.The Journal of experimental medicine · 2010Article
- Early diagnosis of systemic lupus erythmatosus using ANN models of dsDNA binding antibody sequence data.Bioinformation · 2010Article
- Checkpoints in lymphocyte development and autoimmune disease.Nature immunology · 2010Review
- Genetic control of DH reading frame and its effect on B-cell development and antigen-specifc antibody production.Critical reviews in immunology · 2010Review
- Selective recognition of a DNA G-quadruplex by an engineered antibody.Biochemistry · 2008Article
- Specific recognition of a dsDNA sequence motif by an immunoglobulin VH homodimer.Protein science : a publication of the Protein Society · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Combinatorial IgG Fab phage display libraries prepared from a systemic lupus erythematosus (SLE) donor and a healthy donor were affinity selected against human placental DNA. Human monoclonal antibody Fab fragments specific for DNA were isolated from both libraries, although Fabs of the highest affinity were isolated only from the lupus library. Generally, apparent affinities of the Fabs for human placental DNA, purified double-stranded DNA, and denatured DNA were approximately equivalent. Surface plasmon resonance indicated Fab binding constants for a double-stranded oligodeoxynucleotide of 0.2-1.3 x 10(8) M-1. The higher-affinity Fabs, as ranked by binding to human placental DNA or to the oligonucleotide probe, tested positive in the Crithidia luciliae assay commonly used in the diagnosis of SLE, and interestingly the genes encoding the heavy-chain variable regions of these antibodies displayed evidence of only minimal somatic hypermutation. The heavy chains of the SLE Fabs were characterized by a predominance of basic residues toward the N terminus of complementarity-determining region 3 (CDR3). The crucial role of heavy-chain CDR3 (HCDR3) in high-affinity DNA recognition was suggested by the creation of DNA binding in an unrelated antibody by HCDR3 transplantation from SLE antibodies. We propose that high-affinity DNA-binding antibodies can arise in SLE without extensive somatic hypermutation in the variable-region genes because of the expression of inappropriate HCDR3s.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.