ArticleJournal of virology1995
Transcriptional trans activation by human immunodeficiency virus type 1 Tat requires specific coactivators that are not basal factors.
Article in Journal of virology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- HIV Expression in Infected T Cell Clones.Viruses · 2024Review
- Transcription elongation regulator 1 (TCERG1) regulates competent RNA polymerase II-mediated elongation of HIV-1 transcription and facilitates efficient viral replication.Retrovirology · 2013Article
- Regulation of P-glycoprotein by human immunodeficiency virus-1 in primary cultures of human fetal astrocytes.Journal of neuroscience research · 2011Article
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- Article
- Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing.Molecular and cellular biology · 2007Article
- Fox-2 mediates epithelial cell-specific fibroblast growth factor receptor 2 exon choice.Molecular and cellular biology · 2006Article
- COUP-TF interacting protein 2 represses the initial phase of HIV-1 gene transcription in human microglial cells.Nucleic acids research · 2005Article
- Spt5 cooperates with human immunodeficiency virus type 1 Tat by preventing premature RNA release at terminator sequences.Molecular and cellular biology · 2002Article
- Regulation of HIV-1 transcription.Gene expression · 1999Review
- PITALRE, the catalytic subunit of TAK, is required for human immunodeficiency virus Tat transactivation in vivo.Journal of virology · 1998Article
- TAR RNA decoys inhibit tat-activated HIV-1 transcription after preinitiation complex formation.Nucleic acids research · 1997Article
- CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription.Molecular and cellular biology · 1997Article
- Inhibition of human immunodeficiency virus type 1 and type 2 Tat function by transdominant Tat protein localized to both the nucleus and cytoplasm.Journal of virology · 1996Article
- Trans-activation by human immunodeficiency virus Tat protein requires the C-terminal domain of RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- A small circular TAR RNA decoy specifically inhibits Tat-activated HIV-1 transcription.Nucleic acids research · 1996Article
- Activation of transcription factor NF-kappaB by the Tat protein of human immunodeficiency virus type 1.Journal of virology · 1996Article
- Sp1 transcription factor is required for in vitro basal and Tat-activated transcription from the human immunodeficiency virus type 1 long terminal repeat.Journal of virology · 1995Article
- Interactions of thyroid hormone receptor with the human immunodeficiency virus type 1 (HIV-1) long terminal repeat and the HIV-1 Tat transactivator.Journal of virology · 1995Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of human immunodeficiency virus type 1 (HIV-1) genes is regulated by the trans activator Tat. Tat exerts its effects by increasing the rate of transcription, but the mechanism by which it does so is still unknown. To study the cellular factors required for Tat trans activation, we have expressed functional Gst-Tat fusion protein and used it to construct affinity columns. Our findings are as follows. (i) A Gst-Tat affinity matrix depleted HeLa nuclear extracts of a factor(s) required for Tat function. A Tat mutant bearing the missense mutation lysine to alanine at position 41 was incapable of this depletion. (ii) Tat trans activation was recovered by addition of unfractionated nuclear extract, the 0.5 M KCl elution fraction from the Tat affinity column, or sedimentation gradient fractions of HeLa extracts. The activity from the gradients sedimented with an apparent molecular mass of 200 kDa. (iii) Tat trans activation could not be recovered by use of recombinant human TATA-binding protein or partially purified TFIID. (iv) trans activation by Tat was blocked by heating of the nuclear extract under conditions in which basal transcription was not decreased. Our data demonstrate for the first time the existence of unique Tat coactivators distinct from factors required for general basal transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.