ArticleJournal of virology1995
Replication in vitro and in vivo of an equine infectious anemia virus mutant deficient in dUTPase activity.
Article in Journal of virology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 99 citations in OpenAlex.
- Review
- Structural Insight into African Swine Fever Virus dUTPase Reveals a Novel Folding Pattern in the dUTPase Family.Journal of virology · 2020Article
- Deficient uracil base excision repair leads to persistent dUMP in HIV proviruses during infection of monocytes and macrophages.PloS one · 2020Article
- Residues 28 to 39 of the Extracellular Loop 1 of Chicken NaJournal of virology · 2018Article
- Article
- dUTPase: the frequently overlooked enzyme encoded by many retroviruses.Retrovirology · 2015Review
- The dUTPase-related gene of bovine immunodeficiency virus is critical for viral replication, despite the lack of dUTPase activity of the encoded protein.Retrovirology · 2014Article
- Uracil DNA glycosylase initiates degradation of HIV-1 cDNA containing misincorporated dUTP and prevents viral integration.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- HIV DNA is heavily uracilated, which protects it from autointegration.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Genomic analysis of an effective lentiviral vaccine-attenuated equine infectious anemia virus vaccine EIAV FDDV13.Virus genes · 2010Article
- EIAV S2 enhances pro-inflammatory cytokine and chemokine response in infected macrophages.Virology · 2010Article
- An EIAV field isolate reveals much higher levels of subtype variability than currently reported for the equine lentivirus family.Retrovirology · 2009Article
- Replication of equine infectious anemia virus in engineered mouse NIH 3T3 cells.Journal of virology · 2009Article
- Article
- Uracil within DNA: an actor of antiviral immunity.Retrovirology · 2008Review
- Mapping of equine lentivirus receptor 1 residues critical for equine infectious anemia virus envelope binding.Journal of virology · 2008Article
- Discerning an effective balance between equine infectious anemia virus attenuation and vaccine efficacy.Journal of virology · 2005Article
- Differential effects of actin cytoskeleton dynamics on equine infectious anemia virus particle production.Journal of virology · 2004Article
- Characterization of RNA elements that regulate gag-pol ribosomal frameshifting in equine infectious anemia virus.Journal of virology · 2003Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
As an important enzyme in DNA synthesis, dUTPase is present in a wide variety of organisms and viruses and has been identified as a component of the equine infectious anemia virus (EIAV) pol gene. The role of EIAV dUTPase, designated DU, in virus replication in vitro and in vivo was investigated with a recently described infectious molecular clone of EIAV. A deletion mutant that was deficient in dUTPase activity was constructed, and its replication kinetics was examined in fetal equine kidney (FEK) cells and primary equine bone marrow macrophage (EBMM) cells. In FEK cells, which are permissive for EIAV replication, the mutant virus replicated as well as the parental virus. In primary cultures of EBMM cells, which are primary targets of EIAV infection in vivo, the DU mutant showed delayed replication kinetics and replicated to a lower extent than did the parental virus. As the multiplicity of infection decreased, the difference between the parental and mutant viruses increased, such that at the lowest multiplicity of infection tested, there was over a 100-fold difference in virus production. The mutant virus was also much less cytopathic. The role of DU in replication in vivo was examined using a Shetland pony model of EIAV infection. Shetland ponies that were infected with the parental and mutant viruses showed transient virus RNA levels in plasma approximately 5 to 10 days postinfection. The peak virus levels in plasma (as measured by a quantitative reverse transcriptase PCR assay) were 10- to 100-fold lower in the mutant virus-infected animals than in the animals infected with the parental virus. However, ponies infected with the mutant virus mounted similar antibody responses despite the marked differences in virus replication. These studies demonstrate that EIAV DU is important for the efficient replication of the virus in macrophages in vitro and in vivo and suggests that variations in the DU sequence could markedly affect the biological and pathogenic properties of EIAV.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.