ArticleJournal of virology1995
Zinc-binding and protein-protein interactions mediated by the polyomavirus large T antigen zinc finger.
Article in Journal of virology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- A Review of Functional Motifs Utilized by Viruses.Proteomes · 2016Review
- Article
- Lessons in signaling and tumorigenesis from polyomavirus middle T antigen.Microbiology and molecular biology reviews : MMBR · 2009Review
- Lessons from polyoma middle T antigen on signaling and transformation: A DNA tumor virus contribution to the war on cancer.Virology · 2009Review
- Activation of CREB/ATF sites by polyomavirus large T antigen.Journal of virology · 2005Article
- Transactivation of E2F-regulated genes by polyomavirus large T antigen: evidence for a two-step mechanism.Molecular and cellular biology · 2004Article
- Identification of domains of the human papillomavirus type 11 E1 helicase involved in oligomerization and binding to the viral origin.Journal of virology · 2000Article
- J domain-independent regulation of the Rb family by polyomavirus large T antigen.Journal of virology · 2000Article
- The polyoma virus T antigen interferes with interferon-inducible gene expression.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The DnaJ domain of polyomavirus large T antigen is required to regulate Rb family tumor suppressor function.Journal of virology · 1997Article
- Phosphorylation sites in polyomavirus large T antigen that regulate its function in viral, but not cellular, DNA synthesis.Journal of virology · 1997Article
- Genetic analysis of polyomavirus large T nuclear localization: nuclear localization is required for productive association with pRb family members.Journal of virology · 1996Article
- Mutations in the zinc-binding motif of the reovirus capsid protein delta 3 eliminate its ability to associate with capsid protein mu 1.Journal of virology · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Polyomavirus large tumor antigen (LT) contains a potential C2H2 zinc binding element between residues 452 and 472. LT also contains a third histidine in this region, conserved among the polyomavirus LTs. Synthetic peptides of this region bound a single atom of zinc, as determined by spectroscopic analysis. Blotting experiments also showed that fusion proteins containing the element, as well as full-length LT, bound 65Zn. Polyomavirus middle T and small T antigens also bound zinc in the blotting assay. Site-directed mutagenesis showed the importance of this element in LT. Point mutations in four of the conserved residues (C-452, C-455, H-465, and H-469) blocked the ability of LT to function in viral DNA replication, while mutation of H-472-->L decreased replication to 1/30th that of the wild type. Point mutations in intervening residues tested had little effect on replication. Mutants resulting from mutations in the conserved cysteine or histidine residues retained the ability to bind origin DNA. However, they did show a defect in self-association. Because double-hexamer formation is involved in DNA replication, this deficiency is sufficient to explain the defect in replication. Mutants created by point mutations of the coordinating residues were also deficient in replication-associated phosphorylations.
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