ArticleThe Journal of clinical investigation1995
Detection and expression of a cDNA clone that encodes a polypeptide containing two inhibitory domains of human calpastatin and its recognition by rheumatoid arthritis sera.
Article in The Journal of clinical investigation, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 55 citations in OpenAlex.
- Review
- Redox-Mediated Carbamylation As a Hapten Model Applied to the Origin of Antibodies to Modified Proteins in Rheumatoid Arthritis.Antioxidants & redox signaling · 2022Review
- Recent Advances in Research Regarding Autoantibodies in Connective Tissue Diseases and Related Disorders.Internal medicine (Tokyo, Japan) · 2019Review
- Posttranslational Protein Modification in the Salivary Glands of Sjögren's Syndrome Patients.Autoimmune diseases · 2013Article
- Article
- Autoantibodies directed against the protease inhibitor calpastatin in psoriasis.Clinical and experimental immunology · 2005Article
- Update on autoantibodies in rheumatoid arthritis.Current rheumatology reports · 2004Review
- Rheumatoid arthritis specific anti-Sa antibodies target citrullinated vimentin.Arthritis research & therapy · 2004Article
- Autoantibody systems in rheumatoid arthritis: specificity, sensitivity and diagnostic value.Arthritis research · 2002Review
- Cloning of novel soluble gp130 and detection of its neutralizing autoantibodies in rheumatoid arthritis.The Journal of clinical investigation · 2000Article
- In the rheumatoid pannus, anti-filaggrin autoantibodies are produced by local plasma cells and constitute a higher proportion of IgG than in synovial fluid and serum.Clinical and experimental immunology · 2000Article
- Tissue-associated autoantigens in rheumatoid arthritis. Tissue-antigens detected by autoantibodies in synovial fluid and sera of RA patients.Clinical reviews in allergy & immunology · 2000Review
- Insights into rheumatoid arthritis derived from the Sa immune system.Arthritis research · 2000Review
- Rheumatoid arthritis associated autoantibodies in patients with synovitis of recent onset.Arthritis research · 2000Article
- Serum samples of patients with rheumatoid arthritis contain a specific autoantibody to "denatured" aldolase A in the osteoblast-like cell line, MG-63.Annals of the rheumatic diseases · 1999Article
- The challenge of early synovitis: multiple pathways to a common clinical syndrome.Arthritis research · 1999Review
- Antibodies against a novel nucleolar and cytoplasmic antigen (p105-p42) present in the sera of patients with a subset of rheumatoid arthritis (RA) with signs of scleroderma.Clinical and experimental immunology · 1998Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RA is the most frequent and most destructive inflammatory arthropathy. Rheumatoid factors, in spite of their lack of disease specificity, are important serological markers for RA and appear important in its immunopathogenesis as well. In search of more disease-specific autoimmune systems, we have screened a human placenta lambda gt11 cDNA expression library using selected sera from patients with classical erosive RA. We have identified one clone (RA-1) that is recognized by three of five screening sera. The 950-bp insert shows a complete nucleotide sequence homology to the cDNA encoding the two COOH-terminal domains of calpastatin. The deduced open reading frame of the RA-1 cDNA predicts a 284-amino acid protein, with a calculated mol wt of 35.9 kD. Calpastatin is the natural inhibitor of calpains, which are members of the cysteine proteinases recently implicated in joint destruction in rheumatic diseases. The two domains encoded by the RA-1 clone each contain the structural features associated with the inhibitory activity of human calpastatin. By Western blotting, 45.5% or 21/44 RA sera specifically recognized both the fusion and the cleaved recombinant protein. This is in contrast to 4.7% (2/43) in nonrheumatoid sera and 0/10 in normal sera. Anticalpastatin autoantibodies could represent a disease-associated marker in chronic erosive arthritis of the rheumatoid type and could hypothetically play a dual pathogenic role, directly via an immune interference and indirectly through an immune complex mechanism.
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