ReviewClinical microbiology reviews1995
DNA probes and PCR for diagnosis of parasitic infections.
Review in Clinical microbiology reviews, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 152 citations in OpenAlex.
- ParaRef: a decontaminated reference database for parasite detection in ancient and modern metagenomic datasets.Genome biology · 2025Article
- The Power of Old Hats: Rediscovering Inosine-EpPCR to Create Starting Libraries for Whole-Cell-SELEX.Biosensors · 2025Article
- Genotyping of human isolates from human toxoplasmosis outbreak: Restriction Fragment Length Polymorphism and Sanger Sequencing.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- A colorimetric strategy based on dynamic chemistry for direct detection of Trypanosomatid species.Scientific reports · 2019Article
- Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid.BMC infectious diseases · 2019Article
- Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids.Parasites & vectors · 2018Article
- Molecular diagnosis of Toxoplasma gondii infection in Libya.BMC infectious diseases · 2016Article
- Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.Clinical microbiology reviews · 2014Review
- Advances in diagnosis of respiratory diseases of small ruminants.Veterinary medicine international · 2014Review
- Foll Detection of Toxoplasma gondii in sheep and goats blood samples by PCR-RFLP in Urmia.Veterinary research forum : an international quarterly journal · 2013Article
- Universal PCR assays for the differential detection of all Old World Leishmania species.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- Molecular diagnosis of Toxoplasma gondii infection in cerebrospinal fluid from AIDS patients.Cerebrospinal fluid research · 2009Article
- The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.Parasitology research · 2008Review
- Diagnosis of cerebral toxoplasmosis in AIDS patients in Brazil: importance of molecular and immunological methods using peripheral blood samples.Journal of clinical microbiology · 2005Article
- Detection and quantification of Plasmodium falciparum in blood samples using quantitative nucleic acid sequence-based amplification.Journal of clinical microbiology · 2000Article
- Use of PCR in diagnosis of human american tegumentary leishmaniasis in Rio de Janeiro, Brazil.Journal of clinical microbiology · 1999Article
- Molecular techniques for detection, species differentiation, and phylogenetic analysis of microsporidia.Clinical microbiology reviews · 1999Review
- Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis.Journal of clinical microbiology · 1997Article
- Detection of animal pathogens by using the polymerase chain reaction (PCR).Veterinary journal (London, England : 1997) · 1997Review
- Trichinosis.Brain pathology (Zurich, Switzerland) · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA probe and PCR-based assays to identify and detect parasites are technically complex; however, they have high sensitivity, directly detect parasites independent of the immunocompetence or previous clinical history of the patient, and can distinguish between organisms that are morphologically similar. Diagnosis of parasites is often based on direct detection by microscopy, which is insensitive and laborious and can lack specificity. Most PCR-based assays were more sensitive than DNA probe assays. The development of PCR-based diagnostic assays requires multiple steps following the initial selection of oligonucleotide primers and reporter probe. Generally, the ability to detect the DNA of one parasite was attained by PCR; however, advances in the preparation of samples for PCR (extraction of DNA while removing PCR inhibitors) will be required to achieve that sensitivity with human specimens. Preliminary PCR systems have been developed for many different parasites, yet few have been evaluated with a large number of clinical specimens and/or under field conditions. Those evaluations are essential for determination of clinical and field utility and performance and of the most appropriate application of the assay. Several situations in which PCR-based diagnosis will result in epidemiologic, medical, or public health advances have been identified.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.