ArticleThe Biochemical journal1995
Detection of acyl-CoA-binding protein in human red blood cells and investigation of its role in membrane phospholipid renewal.
Article in The Biochemical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 64 citations in OpenAlex.
- RBC-GEM: A genome-scale metabolic model for systems biology of the human red blood cell.PLoS computational biology · 2025Article
- From benzodiazepines to fatty acids and beyond: revisiting the role of ACBP/DBI.Trends in endocrinology and metabolism: TEM · 2021Review
- Deciphering the roles of acyl-CoA-binding proteins in plant cells.Protoplasma · 2016Review
- Ligand binding to the ACBD6 protein regulates the acyl-CoA transferase reactions in membranes.Journal of lipid research · 2015Article
- The acyl-CoA binding protein is required for normal epidermal barrier function in mice.Journal of lipid research · 2012Article
- Disruption of the acyl-CoA-binding protein gene delays hepatic adaptation to metabolic changes at weaning.The Journal of biological chemistry · 2011Article
- Downregulation of PPARs and SREBP by acyl-CoA-binding protein overexpression in transgenic rats.Pflugers Archiv : European journal of physiology · 2008Article
- Mammalian acyl-CoA:lysophosphatidylcholine acyltransferase enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Acyl-CoA binding proteins; structural and functional conservation over 2000 MYA.Molecular and cellular biochemistry · 2007Article
- ACBP--a PPAR and SREBP modulated housekeeping gene.Molecular and cellular biochemistry · 2006Article
- Intracellular calcium changes induced by the endozepine triakontatetraneuropeptide in human polymorphonuclear leukocytes: role of protein kinase C and effect of calcium channel blockers.Cell communication and signaling : CCS · 2004Article
- Membrane localization of Arabidopsis acyl-CoA binding protein ACBP2.Plant molecular biology · 2003Article
- Overexpression of acyl-coA binding protein and its effects on the flux of free fatty acids in McA-RH 7777 cells.Lipids · 2001Article
- The malonyl-CoA-long-chain acyl-CoA axis in the maintenance of mammalian cell function.The Biochemical journal · 1999Review
- Pseudo-enzymatic S-acylation of a myristoylated yes protein tyrosine kinase peptide in vitro may reflect non-enzymatic S-acylation in vivo.The Biochemical journal · 1998Article
- Role of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling.The Biochemical journal · 1997Review
- Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Acyl-CoA-binding protein (ACBP) has been identified in a number of tissues and shown to affect the intracellular distribution and utilization of acyl-CoA. We have detected ACBP in the cytosol but not the membrane of human red blood cells and, using an e.l.i.s.a. with antibodies prepared against human liver ACBP, found that its concentration was 0.5 microM. To investigate the role of ACBP in human red blood cells, we added purified human liver ACBP and radiolabelled acyl-CoA to isolated membranes from these cells. ACBP prevented high concentrations of acyl-CoA from binding to the membrane but could not keep the acyl-CoA in the aqueous phase at low concentrations. This suggested the presence of a pool in the membrane with a binding affinity for acyl-CoA that was greater than that of ACBP for acyl-CoA. In the presence of lysophospholipid, this membrane-bound pool of acyl-CoA was rapidly used as a substrate by acyl-CoA:lysophospholipid acyltransferase (LAT) to generate phospholipid from lysophospholipid. We also found that ACBP-bound acyl-CoA was preferred over free acyl-CoA as a substrate by LAT. These results are the first documentation that human red blood cells contain ACBP and that this protein can affect the utilization of acyl-CoA in plasma membranes of these cells. The interactions between acyl-CoA, ACBP and the membrane suggest that there are several pools of acyl-CoA in the human red blood cell and that ACBP may have a role in regulating their distribution and fate.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.