ArticleThe Journal of cell biology1995
A talin homologue of Dictyostelium rapidly assembles at the leading edge of cells in response to chemoattractant.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 100 citations in OpenAlex.
- Talin force coupling underlies eukaryotic cell-substrate adhesion.Nature communications · 2025Article
- Copine C plays a role in adhesion and streaming inCell adhesion & migration · 2024Article
- Adhesion ofFrontiers in cell and developmental biology · 2022Review
- Neuronal Nitric Oxide Synthase (nNOS) in Neutrophils: An Insight.Reviews of physiology, biochemistry and pharmacology · 2021Review
- A recombinant antibody toolbox for Dictyostelium discoideum.BMC research notes · 2020Article
- Drosophila vinculin is more harmful when hyperactive than absent, and can circumvent integrin to form adhesion complexes.Journal of cell science · 2016Article
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- Talin couples the actomyosin cortex to the plasma membrane during rear retraction and cytokinesis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Uncovering a role for the tail of the Dictyostelium discoideum SadA protein in cell-substrate adhesion.Eukaryotic cell · 2011Article
- Overlapping functions of the two talin homologues in Dictyostelium.Eukaryotic cell · 2008Article
- Talin influences the dynamics of the myosin VII-membrane interaction.Molecular biology of the cell · 2007Article
- Evidence that talin alternative splice variants from Ciona intestinalis have different roles in cell adhesion.BMC cell biology · 2006Article
- An adhesion molecule in free-living Dictyostelium amoebae with integrin beta features.EMBO reports · 2006Article
- The bundling activity of vasodilator-stimulated phosphoprotein is required for filopodium formation.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Cellular distribution and functions of wild-type and constitutively activated Dictyostelium PakB.Molecular biology of the cell · 2005Article
- Phg2, a kinase involved in adhesion and focal site modeling in Dictyostelium.Molecular biology of the cell · 2004Article
- Talin B is required for force transmission in morphogenesis of Dictyostelium.The EMBO journal · 2004Article
- SadA, a novel adhesion receptor in Dictyostelium.The Journal of cell biology · 2002Article
- Further characterization of the interaction between the cytoskeletal proteins talin and vinculin.The Biochemical journal · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In an attempt to identify unknown actin-binding proteins in cells of Dictyostelium discoideum that may be involved in the control of cell motility and chemotaxis, monoclonal antibodies were raised against proteins that had been enriched on an F-actin affinity matrix. One antibody recognized a protein distinguished by its strong accumulation at the tips of filopods. These cell-surface extensions containing a core of bundled actin filaments are rapidly protruded and retracted by cells in the growth-phase stage. The protein of 269 kD turned out to resemble mouse fibroblast talin (Rees et al., 1990) in its primary structure. The fit is best among the first 400-amino acid residues of the NH2-terminal region where identity between the two proteins is 44% and the last 200-amino acid residues of the COOH-terminal region with 36% identity. In the elongated cells of the aggregation stage the Dictyostelium talin is accumulated at the entire front where also F-actin is enriched. Since this protein exists in a soluble state in the cytoplasm, mechanisms are predicted that cause accumulation at sites of the cell where a front is established. Evidence for receptor-mediated accumulation was obtained by local stimulation of cells with cAMP. When a new front was induced by the chemoattractant, the talin accumulated there within half a minute, indicating a signal cascade in Dictyostelium responsible for assembly of the talin beneath sites of the plasma membrane where chemoattractant receptors are strongly activated. The ordered assembly of the talin homologue together with actin and a series of other proteins is considered to play a key role in chemotactic orientation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.