ArticleThe Plant cell1995
Reconstitution of Arabidopsis casein kinase II from recombinant subunits and phosphorylation of transcription factor GBF1.
Article in The Plant cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 97 citations in OpenAlex.
- A pervasive phosphorylation cascade modulation of plant transcription factors in response to abiotic stress.Planta · 2023Review
- Calmodulin7: recent insights into emerging roles in plant development and stress.Plant molecular biology · 2021Review
- Phosphorylation of p23-1 cochaperone by protein kinase CK2 affects root development in Arabidopsis.Scientific reports · 2019Article
- A Casein Kinase II Phosphorylation Site in AtYY1 Affects Its Activity, Stability, and Function in the ABA Response.Frontiers in plant science · 2017Article
- Phosphorylation Affects DNA-Binding of the Senescence-Regulating bZIP Transcription Factor GBF1.Plants (Basel, Switzerland) · 2015Article
- Emerging roles of protein kinase CK2 in abscisic acid signaling.Frontiers in plant science · 2015Review
- Functional relationships of phytoene synthase 1 alleles on chromosome 7A controlling flour colour variation in selected Australian wheat genotypes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- G-Box binding factor1 reduces CATALASE2 expression and regulates the onset of leaf senescence in Arabidopsis.Plant physiology · 2010Article
- Phosphorylation of maize eukaryotic translation initiation factor 5A (eIF5A) by casein kinase 2: identification of phosphorylated residue and influence on intracellular localization of eIF5A.The Journal of biological chemistry · 2010Article
- FW2.2 and cell cycle control in developing tomato fruit: a possible example of gene co-option in the evolution of a novel organ.Plant molecular biology · 2006Article
- Pea DNA topoisomerase I is phosphorylated and stimulated by casein kinase 2 and protein kinase C.Plant physiology · 2003Article
- Mutation of capsid protein phosphorylation sites abolishes cauliflower mosaic virus infectivity.Journal of virology · 2002Article
- Phase-specific circadian clock regulatory elements in Arabidopsis.Plant physiology · 2002Article
- Distinctive features of plant protein kinase CK2.Molecular and cellular biochemistry · 2001Article
- Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the alpha subunit of protein kinase CK2.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- A nuclear casein kinase 2 activity is involved in early events of transcriptional activation induced by salicylic acid in tobacco.Plant physiology · 2001Article
- Caleosins: Ca2+-binding proteins associated with lipid bodies.Plant molecular biology · 2000Article
- HY5 stability and activity in arabidopsis is regulated by phosphorylation in its COP1 binding domain.The EMBO journal · 2000Article
- Characterization, subcellular localization and nuclear targeting of casein kinase 2 from Zea mays.Plant molecular biology · 1999Article
- Antisense expression of the CK2 alpha-subunit gene in Arabidopsis. Effects on light-regulated gene expression and plant growth.Plant physiology · 1999Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In contrast to the well-defined tetrameric structure of animal and yeast casein kinase II (CKII), plant CKII is found in two forms: a monomeric form and an oligomeric form whose subunit composition is not well defined. The Arabidopsis homologs of the catalytic subunit alpha (CKA1) and the regulatory subunit beta (CKB1) of CKII were expressed in Escherichia coli to examine their ability to form complexes, the effect of CKB1 on the catalytic activity, and the relationship of the recombinant enzymes to those isolated from plant material. Both subunits were found mainly in the inclusion body fraction in the bacterial expression strains, and they were solubilized and renatured with the recovery of catalytic (CKA1) and stimulatory (CKB1) activities. The combination of purified CKA1 and CKB1 proteins resulted in up to 100-fold stimulation of casein kinase activity compared with the CKA1 activity alone, showing that CKB1 has biochemical properties similar to those of the beta subunit from animals. CKA1 and CKB1 spontaneously assembled into a tetrameric complex, CKA1(2)CKB12, which had properties very similar to those of the oligomeric CKII form isolated from broccoli. However, the properties of the catalytic subunit CKA1 alone differed from those of the broccoli monomeric form of CKII-like activity. Phosphorylation of transcription factor GBF1 with the reconstituted CKA1(2)CKB1(2) enzyme resulted in stimulation of its DNA binding activity and retardation of the protein-DNA complex; these results are identical to those obtained previously with isolated nuclear CKII from broccoli.
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