ArticleMolecular biology of the cell1994
Splicing components are excluded from the transcriptionally inactive XY body in male meiotic nuclei.
Article in Molecular biology of the cell, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
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- MRNIP interacts with sex body chromatin to support meiotic progression, spermatogenesis, and male fertility in mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- The Dispensable Roles of X-LinkedFrontiers in genetics · 2021Article
- Ubiquitin Ligase Huwe1 Modulates Spermatogenesis by Regulating Spermatogonial Differentiation and Entry into Meiosis.Scientific reports · 2017Article
- Unique sex chromosome systems in Ellobius: How do male XX chromosomes recombine and undergo pachytene chromatin inactivation?Scientific reports · 2016Article
- CTCF contributes in a critical way to spermatogenesis and male fertility.Scientific reports · 2016Article
- Human male meiotic sex chromosome inactivation.PloS one · 2012Article
- Chromatin configuration and epigenetic landscape at the sex chromosome bivalent during equine spermatogenesis.Chromosoma · 2011Article
- Gene duplication and the genome distribution of sex-biased genes.International journal of evolutionary biology · 2011Article
- RNF8-dependent histone modifications regulate nucleosome removal during spermatogenesis.Developmental cell · 2010Article
- Poly(ADP-ribose) polymerase-2 contributes to the fidelity of male meiosis I and spermiogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Role for centromeric heterochromatin and PML nuclear bodies in the cellular response to foreign DNA.Molecular and cellular biology · 2006Article
- Differential expression of sex-linked and autosomal germ-cell-specific genes during spermatogenesis in the mouse.Human molecular genetics · 2005Article
- The mouse juvenile spermatogonial depletion (jsd) phenotype is due to a mutation in the X-derived retrogene, mUtp14b.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
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Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of the effect of programmed cessation of transcription in a large nuclear domain, on the distribution of elements of the pre-mRNA splicing machinery, is the main aim of this paper. To this end, we took advantage of the nuclear partitioning of mouse spermatocytes early in meiosis into autosomal transcribing and XY nontranscribing compartments. This system also allows to extend this study to stages in sperm differentiation that are accompanied by reduction and eventual cessation of transcription. We show by indirect immunofluorescence in spermatogenetic cells that 1) fluorescent signals of the pre-mRNA splicing factors SF53/4 and SC35, of the Sm antigens, and of RNA polymerase II, are largely absent from the nontranscribing, X-inactivated compartment, but are abundantly present in the transcribing autosomal compartment and 2) the presence, gradual reduction, and absence of transcriptive activity in nuclei undergoing the sperm formation sequence are positively correlated with the fluorescence patterns of the antibodies against SF53/4, SC35, and the Sm antigens. These data suggest that cessation of transcription during spermatogenesis is accompanied by exclusion of the splicing machinery from nontranscribing chromatin to its vicinity.
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