ArticleBiophysical journal1995
Comparison of quantitative calcium flux through NMDA, ATP, and ACh receptor channels.
Article in Biophysical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 159 citations in OpenAlex.
- Nano-organization of synaptic calcium signaling.Biochemical Society transactions · 2024Review
- Article
- Integrating Bioelectrical Currents and CaBioelectricity · 2020Review
- Autocrine, paracrine and necrotic NMDA receptor signalling in mouse pancreatic neuroendocrine tumour cells.Open biology · 2017Article
- The habenulo-interpeduncular pathway in nicotine aversion and withdrawal.Neuropharmacology · 2015Review
- Quantifying Ca2+ current and permeability in ATP-gated P2X7 receptors.The Journal of biological chemistry · 2015Article
- Principles and properties of ion flow in P2X receptors.Frontiers in cellular neuroscience · 2014Review
- TRPC6 inhibited NMDA current in cultured hippocampal neurons.Neuromolecular medicine · 2013Article
- Neuroprotection of rat retinal ganglion cells mediated through alpha7 nicotinic acetylcholine receptors.Neuroscience · 2013Article
- Synaptic neurotransmitter-gated receptors.Cold Spring Harbor perspectives in biology · 2012Review
- Purine receptor-mediated endocannabinoid production and retrograde synaptic signalling in the cerebellar cortex.British journal of pharmacology · 2011Article
- Evaluation of Ca2+ permeability of nicotinic acetylcholine receptors in hypothalamic histaminergic neurons.Acta biochimica et biophysica Sinica · 2010Article
- Acute nicotine reduces brain arachidonic acid signaling in unanesthetized rats.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2009Article
- Decremental response to high-frequency trains of acetylcholine pulses but unaltered fractional Ca2+ currents in a panel of "slow-channel syndrome" nicotinic receptor mutants.The Journal of general physiology · 2009Article
- Acidic amino acids impart enhanced Ca2+ permeability and flux in two members of the ATP-gated P2X receptor family.The Journal of general physiology · 2007Article
- Excitatory effect of ATP on rat area postrema neurons.Purinergic signalling · 2006Article
- Biophysics of P2X receptors.Pflugers Archiv : European journal of physiology · 2006Review
- alpha3alpha5beta2-Nicotinic acetylcholine receptor contributes to the wound repair of the respiratory epithelium by modulating intracellular calcium in migrating cells.The American journal of pathology · 2006Article
- Ca2+ permeability of nicotinic acetylcholine receptors from rat dorsal root ganglion neurones.The Journal of physiology · 2005Article
- A modeling environment with three-dimensional morphology, A-Cell-3D, and Ca2+ dynamics in a spine.Neuroinformatics · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
NMDA receptors, ATP receptors, and nicotinic ACh receptors respond to agonist by undergoing conformational changes that open weakly selective cationic channels that are permeable to calcium. We determined the fraction of the current carried by calcium by simultaneously measuring membrane current using whole-cell patch-clamp techniques and intracellular Ca2+ using the fluorescent indicator Fura-2. The Fura-2 response to free Ca2+ was calibrated individually for each cell. Two different calibration methods are compared: one uses voltage-activated Ca2+ channels, and the other uses the same ligand-gated channels that are being tested but in a pure Ca2+ solution. The two methods give quantitatively different results. The method using pure Ca2+ currents through ligand-gated channels calibrates the Fura-2 signal through the same influx pathway that generates the test response, thus controlling for the distribution of channels and ensuring a similar interaction between the incoming Ca2+ and Fura-2. In a physiologic solution containing 2.5 mM Ca2+ at a holding potential of -50 mV, the percentage of inward current carried by Ca2+ through NMDA receptors in hippocampal neurons is 12.4%. By comparison, in sympathetic neurons the percentage of current carried by Ca2+ through neuronal nAChRs is 4.7%, and through ATP-activated purinergic receptors it is 6.5%. These percentages can be used to estimate the amount of Ca2+ entry through these receptors during synaptic activation, but care must be exercised in considering the many subtypes of each receptor.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.