ArticleBiophysical journal1994
Inactivation of L-type Ca channels in embryonic chick ventricle cells: dependence on the cytoskeletal agents colchicine and taxol.
Article in Biophysical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 109 citations in OpenAlex.
- The L-type Ca(2+) channel facilitates abnormal metabolic activity in the cTnI-G203S mouse model of hypertrophic cardiomyopathy.The Journal of physiology · 2016Article
- Colchicine modulates calcium homeostasis and electrical property of HL-1 cells.Journal of cellular and molecular medicine · 2016Article
- Docetaxel modulates the delayed rectifier potassium current (IK) and ATP-sensitive potassium current (IKATP) in human breast cancer cells.The Journal of membrane biology · 2015Article
- THE INHIBITORY EFFECT OF PACLITAXEL ON (Kv2.1) K+ CURRENT IN H9c2 CELLS.Fukushima journal of medical science · 2015Article
- Electrical and Mechanical Strategies to Enable Cardiac Repair and Regeneration.IEEE reviews in biomedical engineering · 2015Review
- Impaired functional communication between the L-type calcium channel and mitochondria contributes to metabolic inhibition in the mdx heart.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Mechanical regulation of native and the recombinant calcium channel.Cell calcium · 2013Article
- A new calpain inhibitor protects left ventricular dysfunction induced by mild ischemia-reperfusion in in situ rat hearts.The journal of physiological sciences : JPS · 2013Article
- Mechanotransduction: the role of mechanical stress, myocyte shape, and cytoskeletal architecture on cardiac function.Pflugers Archiv : European journal of physiology · 2011Review
- Colchicine modulates oxidative stress in serum and leucocytes from remission patients with Family Mediterranean Fever through regulation of Ca²+ release and the antioxidant system.The Journal of membrane biology · 2011Article
- The multiple roles of myelin protein genes during the development of the oligodendrocyte.ASN neuro · 2010Review
- Lysophosphatidyl choline modulates mechanosensitive L-type Ca2+ current in circular smooth muscle cells from human jejunum.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Rescue of Ca2+ overload-induced left ventricular dysfunction by targeted ablation of phospholamban.American journal of physiology. Heart and circulatory physiology · 2009Article
- Tubulin as a binding partner of the heag2 voltage-gated potassium channel.The Journal of membrane biology · 2008Article
- Extracellular matrix, mechanotransduction and structural hierarchies in heart tissue engineering.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2007Review
- Convergent regulation of skeletal muscle Ca2+ channels by dystrophin, the actin cytoskeleton, and cAMP-dependent protein kinase.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Contraction augments L-type Ca2+ currents in adherent guinea-pig cardiomyocytes.The Journal of physiology · 2004Article
- Cytochalasin D reduces Ca2+ currents via cofilin-activated depolymerization of F-actin in guinea-pig cardiomyocytes.The Journal of physiology · 2001Article
- Cardiocyte cytoskeleton in hypertrophied myocardium.Heart failure reviews · 2000Review
- Voltage- and calcium-dependent inactivation of calcium channels in Lymnaea neurons.The Journal of general physiology · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
This article shows that colchicine and taxol strongly influence the kinetics of L-type Ca channels in intact cardiac cells, and it suggests a mechanism for this action. It is known that colchicine disassociates microtubules into tubulin, and that taxol stabilizes microtubules. We have found that colchicine increases the probability that Ca channels are in the closed state and that taxol increases the probability they are in the open state. Moreover, taxol lengthens the mean open time of Ca channels. In this regard, taxol is similar to Bay-K 8644; however, Bay K works on inside-out patches, but taxol does not. Neither colchicine nor taxol alters the number of Ca channels in a patch. We have quantified these results as follows. It is known that L-type channels in embryonic chick heart ventricle cells have voltage- and current-dependent inactivation. In 10 mM Ba, channel conductance is linear in the range -10 to 20 mV. The conductance is 12 +/- 1 pS, and the extrapolated reversal potential is 42 +/- 2 mV (n = 3). In cell-attached patches, inactivation depends on the number of channels. One channel (holding at -80 mV and stepping to 0 mV for 500 ms) shows virtually no inactivation. However, three channels inactivate with a time constant of 360 +/- 20 ms (n = 6). In similar patches, colchicine (80 microM for 15 min) decreases the inactivation time constant to 162 +/- 33 ms (n = 4) and taxol (50 microM for 10 min) virtually abolishes inactivation (time constant 812 +/- 265 ms (n = 4)). We suggest that colchicine and taxol affect Ca channels through their action on the cytoskeleton, which in turn regulates the effective concentration of inactivating ions near the mouths of channels. An alternate explanation is that free tubulin interacts directly with Ca channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.