ArticleThe Biochemical journal1993
Reactive oxygen species mediate phorbol ester-regulated tyrosine phosphorylation and phospholipase A2 activation: potentiation by vanadate.
Article in The Biochemical journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 114 citations in OpenAlex.
- Reactive Oxygen Species in the Reproductive System: Sources and Physiological Roles.Advances in experimental medicine and biology · 2022Article
- Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development.Scientific reports · 2017Article
- Contemporary evidence on the physiological role of reactive oxygen species in human sperm function.Journal of assisted reproduction and genetics · 2015Review
- Pituitary Adenylate Cyclase-Activating Polypeptide Reverses Ammonium Metavanadate-Induced Airway Hyperresponsiveness in Rats.Oxidative medicine and cellular longevity · 2015Article
- Pulmonary endothelial cell NOX.American journal of respiratory cell and molecular biology · 2012Review
- The roles of phytochemicals in bronchial asthma.Molecules (Basel, Switzerland) · 2010Review
- Nox proteins in signal transduction.Free radical biology & medicine · 2009Review
- Downstream targets and intracellular compartmentalization in Nox signaling.Antioxidants & redox signaling · 2009Review
- Redox-regulation of Erk1/2-directed phosphatase by reactive oxygen species: role in signaling TPA-induced growth arrest in ML-1 cells.Journal of cellular physiology · 2008Article
- Ras activation in response to phorbol ester proceeds independently of the EGFR via an unconventional nucleotide-exchange factor system in COS-7 cells.The Biochemical journal · 2006Article
- Insulin inhibits platelet-derived growth factor-induced cell proliferation.Molecular biology of the cell · 2005Article
- Involvement of cytosolic phospholipase A2, and the subsequent release of arachidonic acid, in signalling by rac for the generation of intracellular reactive oxygen species in rat-2 fibroblasts.The Biochemical journal · 2000Article
- Agonist-specific alterations in receptor-phospholipase coupling following inactivation of Gi2alpha gene.The Biochemical journal · 1998Article
- Requirement for tyrosine phosphatase during serotonergic neuromodulation by protein kinase C.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- High rates of extracellular superoxide generation by cultured human fibroblasts: involvement of a lipid-metabolizing enzyme.The Biochemical journal · 1996Article
- Biosynthesis of the endogenous cyclic adenosine monophosphate (AMP) antagonist, prostaglandylinositol cyclic phosphate (cyclic PIP), from prostaglandin E and activated inositol polyphosphate in rat liver plasma membranes.Acta diabetologica · 1996Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously shown that vanadate potentiates the activating effect of phorbol ester (TPA) on cellular phospholipase A2 (PLA2) in a pathway dependent on the formation of reactive oxygen species (ROS). Here we evaluate the chain of enzymes (protein kinases and phosphatases) that participate in this process. Treatment of macrophages with vanadate plus TPA led to activation of protein kinase C (PKC) and NADPH oxidase (O2- generation in intact cells), massive cellular protein tyrosine phosphorylation, suppression of protein tyrosine phosphatase (PTP) activity and a sustained activation of protein tyrosine kinase (PTK) and myelin basic protein kinase activity (the latter three enzyme activities were assessed in cell lysates). Inhibition of ROS formation by diphenyleneiodonium (DPI) prevented PTP inhibition, PTK activation and protein tyrosine phosphorylation by vanadate plus TPA. Vanadate plus H2O2 mimicked the effect of vanadate plus TPA on PKC activation, cellular protein tyrosine phosphorylation, PTP and PTK, but their effects were resistant to DPI. Suppression of PKC activity (down-regulation; selective inhibitors) prevented the above-mentioned effects of vanadate plus TPA, but not of vanadate plus H2O2. Collectively, the results show that ROS formation induced by TPA in association with vanadate is essential in the modulation of protein tyrosine phosphorylation and PLA2 activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.